C57BL/6NCya-Myo1fem1/Cya
Common Name:
Myo1f-KO
Product ID:
S-KO-03312
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Myo1f-KO
Strain ID
KOCMP-17916-Myo1f-B6N-VA
Gene Name
Product ID
S-KO-03312
Gene Alias
C330006B10Rik
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Myo1fem1/Cya mice (Catalog S-KO-03312) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000087605
NCBI RefSeq
NM_053214
Target Region
Exon 5~10
Size of Effective Region
~4.3 kb
Detailed Document
Overview of Gene Research
Myo1f, an unconventional long-tailed class Ⅰ myosin, plays significant roles in immune cell functions, including motility, adhesion, and migration, and is involved in multiple immune-related pathways [2,3,7]. It also contributes to antifungal immunity, T-cell activation, and metabolism, and is associated with diseases such as fungal infections, peripheral T-cell lymphoma (PTCL), and allergic reactions [1,4,5]. Genetic models, especially KO/CKO mouse models, are valuable for studying its functions.
Myo1f-deficient mice are more susceptible to systemic Candida albicans infections, indicating its role in promoting antifungal immunity by regulating α-tubulin acetylation [1]. In γδT intraepithelial lymphocytes, Myo1f is essential for adhesion and migration. Its absence affects lymphocyte homing due to reduced CCR9 and α4β7 surface expression [2]. In mast cells, knockdown of Myo1F reduces degranulation induced by IgE cross-linking and MRGPRX2 stimulation, suggesting its role in allergic and pseudoallergic drug reactions [3]. T-cell-specific Myo1f knockout mice exhibit an increased tumor burden and attenuated experimental autoimmune encephalomyelitis (EAE) severity due to impaired T-cell activation [4]. In Aspergillus fumigatus keratitis mouse models, knockdown of Myo1f reduces disease severity, decreases neutrophil and macrophage recruitment, and suppresses the MyD88/NF-κB signaling pathway [6].
In conclusion, Myo1f is crucial for immune cell-related functions and has implications in fungal infections, allergic responses, and PTCL. KO/CKO mouse models have been instrumental in revealing its role in these disease-related biological processes, providing insights into potential therapeutic strategies for these conditions.
References:
1. Sun, Wanwei, Ma, Xiaojian, Wang, Heping, Li, Xiaoxia, Wang, Chenhui. . MYO1F regulates antifungal immunity by regulating acetylation of microtubules. In Proceedings of the National Academy of Sciences of the United States of America, 118, . doi:10.1073/pnas.2100230118. https://pubmed.ncbi.nlm.nih.gov/34301894/
2. Martínez-Vargas, Irving Ulises, Sánchez-Bello, Maria Elena, Miguel-Rodríguez, Carlos Emilio, Santos-Argumedo, Leopoldo, Talamás-Rohana, Patricia. 2023. Myo1f has an essential role in γδT intraepithelial lymphocyte adhesion and migration. In Frontiers in immunology, 14, 1041079. doi:10.3389/fimmu.2023.1041079. https://pubmed.ncbi.nlm.nih.gov/37207213/
3. Navinés-Ferrer, Arnau, Ainsua-Enrich, Erola, Serrano-Candelas, Eva, Olivera, Ana, Martin, Margarita. 2021. MYO1F Regulates IgE and MRGPRX2-Dependent Mast Cell Exocytosis. In Journal of immunology (Baltimore, Md. : 1950), 206, 2277-2289. doi:10.4049/jimmunol.2001211. https://pubmed.ncbi.nlm.nih.gov/33941653/
4. Cui, Zhihui, Wang, Heping, Feng, Xiong, Xiao, Xue, Wang, Chenhui. 2024. MYO1F regulates T-cell activation and glycolytic metabolism by promoting the acetylation of GAPDH. In Cellular & molecular immunology, 22, 176-190. doi:10.1038/s41423-024-01247-6. https://pubmed.ncbi.nlm.nih.gov/39668163/
5. Cortes, Jose R, Filip, Ioan, Albero, Robert, Rabadan, Raul, Palomero, Teresa. . Oncogenic Vav1-Myo1f induces therapeutically targetable macrophage-rich tumor microenvironment in peripheral T cell lymphoma. In Cell reports, 39, 110695. doi:10.1016/j.celrep.2022.110695. https://pubmed.ncbi.nlm.nih.gov/35443168/
6. Liu, Wenting, Yang, Hua, Xu, Qiang, Sun, Xiaoyan, Che, Chengye. 2024. Role of MYO1F in neutrophil and macrophage recruitment and pro-inflammatory cytokine production in Aspergillus fumigatus keratitis. In International immunopharmacology, 142, 113094. doi:10.1016/j.intimp.2024.113094. https://pubmed.ncbi.nlm.nih.gov/39276460/
7. Navinés-Ferrer, Arnau, Ainsua-Enrich, Erola, Serrano-Candelas, Eva, Sayós, Joan, Martin, Margarita. 2019. Myo1f, an Unconventional Long-Tailed Myosin, Is a New Partner for the Adaptor 3BP2 Involved in Mast Cell Migration. In Frontiers in immunology, 10, 1058. doi:10.3389/fimmu.2019.01058. https://pubmed.ncbi.nlm.nih.gov/31143189/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen