C57BL/6JCya-Nfyaem1/Cya
Common Name
Nfya-KO
Product ID
S-KO-03370
Backgroud
C57BL/6JCya
Strain ID
KOCMP-18044-Nfya-B6J-VA
When using this mouse strain in a publication, please cite “Nfya-KO Mouse (Catalog S-KO-03370) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Nfya-KO
Strain ID
KOCMP-18044-Nfya-B6J-VA
Gene Name
Product ID
S-KO-03370
Gene Alias
Cbf-b, SEZ-10, SEZ10
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 17
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000046719
NCBI RefSeq
NM_001110832
Target Region
Exon 5~6
Size of Effective Region
~1.2 kb
Overview of Gene Research
Nfya, also known as nuclear transcription factor Y subunit alpha, is a transcription factor that plays a crucial role in multiple biological processes. It is involved in pathways related to metabolism, cell proliferation, and development. Nfya is important for coordinating metabolism and proliferation in various cell types, and genetic models are valuable for studying its functions [1,2,3].
In mouse fetal heart development, loss of Nfya in cardiomyocytes leads to altered cell composition, reduced proliferation, and impaired mitochondrial metabolism, resulting in cardiac growth defects and embryonic death. Nfya, interacting with cofactor SP2, activates genes linking metabolism and proliferation at the transcription level [1]. In castration-resistant prostate cancer (CRPC), silencing Nfya in androgen-independent cell lines significantly inhibits cell growth and migration. RNA-seq analysis shows that EGR4 is a downstream target of Nfya, and in vivo studies in mouse models demonstrate that Nfya silencing inhibits xenograft tumor growth [2].
In conclusion, Nfya is essential for regulating prenatal cardiac growth, metabolism, and cell proliferation. Studies using Nfya-deficient mouse models have revealed its role in diseases such as CRPC, highlighting its potential as a therapeutic target in these disease areas.
References:
1. Cui, Miao, Bezprozvannaya, Svetlana, Hao, Tian, Bassel-Duby, Rhonda, Olson, Eric N. 2023. Transcription factor NFYa controls cardiomyocyte metabolism and proliferation during mouse fetal heart development. In Developmental cell, 58, 2867-2880.e7. doi:10.1016/j.devcel.2023.10.012. https://pubmed.ncbi.nlm.nih.gov/37972593/
2. Sun, Guijiang, Shao, Yi, Ma, Qianwang, Wang, Haitao, Shang, Zhiqun. 2024. NFYA-mediated promotion of castration-resistant prostate cancer progression through EGR4 regulation. In Discover oncology, 15, 528. doi:10.1007/s12672-024-01392-4. https://pubmed.ncbi.nlm.nih.gov/39367986/
3. Cui, Miao, Wang, Zhaoning, Chen, Kenian, Bassel-Duby, Rhonda, Olson, Eric N. 2020. Dynamic Transcriptional Responses to Injury of Regenerative and Non-regenerative Cardiomyocytes Revealed by Single-Nucleus RNA Sequencing. In Developmental cell, 53, 102-116.e8. doi:10.1016/j.devcel.2020.02.019. https://pubmed.ncbi.nlm.nih.gov/32220304/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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