C57BL/6NCya-Nos3em1/Cya
Common Name
Nos3-KO
Product ID
S-KO-03405
Backgroud
C57BL/6NCya
Strain ID
KOCMP-18127-Nos3-B6N-VA
When using this mouse strain in a publication, please cite “Nos3-KO Mouse (Catalog S-KO-03405) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Nos3-KO
Strain ID
KOCMP-18127-Nos3-B6N-VA
Gene Name
Product ID
S-KO-03405
Gene Alias
eNOS, Nos-3, ecNOS, 2310065A03Rik
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 5
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000030834
NCBI RefSeq
NM_008713
Target Region
Exon 2~13
Size of Effective Region
~6.0 kb
Overview of Gene Research
Nos3, also known as endothelial nitric oxide synthase (eNOS), is a crucial enzyme in the cardiovascular system. It synthesizes nitric oxide (NO) from L-arginine, which serves as an important vasodilator and plays a vital role in maintaining cardiovascular homeostasis. NOS3 expression and activity are regulated at transcriptional, post-transcriptional, and post-translational levels [3].
In a study using adipocyte-specific NOS3 knockout (A-NOS3 KO) mice, despite less weight gain, these mice showed a significant increase in blood pressure after high-fat diet (HFD) feeding, along with exacerbated vascular dysfunction and remodelling. This indicates that NOS3 in adipocytes is essential for maintaining vascular homeostasis, and its dysfunction contributes to obesity-induced vascular remodelling and hypertension [1]. Another study established an ApoE/NOS3 double knockout mouse model. ApoE/NOS3 -/- mice developed hypertensive fundus retinopathy, hypertensive nephropathy, abnormal elevation of serum cholesterol, and atherosclerotic lesions, suggesting it is a useful model for studying hypertension and atherosclerosis [2].
In conclusion, Nos3 is vital for cardiovascular homeostasis. Gene knockout mouse models, such as A-NOS3 KO and ApoE/NOS3 -/- mice, have been instrumental in revealing its role in obesity-related hypertension, vascular remodelling, and atherosclerotic disease. These findings enhance our understanding of the biological functions of Nos3 and its implications in disease pathogenesis.
References:
1. Man, Andy W C, Zhou, Yawen, Reifenberg, Gisela, Xia, Ning, Li, Huige. . Deletion of adipocyte NOS3 potentiates high-fat diet-induced hypertension and vascular remodelling via chemerin. In Cardiovascular research, 119, 2755-2769. doi:10.1093/cvr/cvad164. https://pubmed.ncbi.nlm.nih.gov/37897505/
2. Liu, Ke, Chen, Bangzhu, Zeng, Fanwen, Yan, Jiarong, Zhang, Shouquan. 2022. ApoE/NOS3 Knockout Mice as a Novel Cardiovascular Disease Model of Hypertension and Atherosclerosis. In Genes, 13, . doi:10.3390/genes13111998. https://pubmed.ncbi.nlm.nih.gov/36360235/
3. Oliveira-Paula, Gustavo H, Lacchini, Riccardo, Tanus-Santos, Jose E. 2015. Endothelial nitric oxide synthase: From biochemistry and gene structure to clinical implications of NOS3 polymorphisms. In Gene, 575, 584-99. doi:10.1016/j.gene.2015.09.061. https://pubmed.ncbi.nlm.nih.gov/26428312/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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