C57BL/6JCya-Prdx1em1/Cya
Common Name:
Prdx1-KO
Product ID:
S-KO-03550
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Prdx1-KO
Strain ID
KOCMP-18477-Prdx1-B6J-VA
Gene Name
Product ID
S-KO-03550
Gene Alias
MSP23; NkefA; OSF-3; OSF3; PAG; Paga; PrdxI; PrxI; TDX2; TPxA; Tdpx2; prx1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Prdx1em1/Cya mice (Catalog S-KO-03550) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000135573
NCBI RefSeq
NM_011034.4
Target Region
Exon 3
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Prdx1, short for peroxiredoxin 1, is a member of the peroxidase family. It functions primarily in protecting cells against damage caused by H₂O₂, acting as an antioxidant enzyme. It is also involved in multiple cellular signaling pathways, including those related to NF-κB activation, autophagy, and redox-regulation, which are crucial for maintaining cellular homeostasis and are associated with various biological processes and diseases [1].
In colorectal cancer, PRDX1 knockout mice showed a significant decrease in the number of AOM/DSS-induced colitis-associated CRC, accompanied by downregulation of NRF2 and GPX4. Mechanistically, knockdown of PRDX1 led to ROS-induced mitochondrial dysfunction and lipid peroxidation-induced ferroptosis in CRC cells, suggesting that PRDX1 promotes CRC progression by suppressing CUL3-mediated NRF2 degradation [2]. In glioma cells, radiation-induced IRAK1 binds to PRDX1, preventing its ubiquitination and degradation by E3 ubiquitin ligase HECTD3. Overexpression of PRDX1 reverses the radiotherapy sensitization effect of IRAK1 depletion by diminishing autophagic cell death, indicating the IRAK1-PRDX1 axis as a potential therapeutic target [3].
In conclusion, Prdx1 is essential for antioxidant defense, redox-regulation, and the modulation of multiple signaling pathways. The use of Prdx1 knockout mouse models has provided valuable insights into its role in diseases such as colorectal cancer and glioma, highlighting its potential as a therapeutic target. Understanding Prdx1's functions can help in developing new strategies for treating these diseases.
References:
1. Guan, Xin, Ruan, Yiyin, Che, Xiaoxia, Feng, Weiwei. 2023. Dual role of PRDX1 in redox-regulation and tumorigenesis: Past and future. In Free radical biology & medicine, 210, 120-129. doi:10.1016/j.freeradbiomed.2023.11.009. https://pubmed.ncbi.nlm.nih.gov/37977211/
2. Song, Yujia, Wang, Xiaohui, Sun, Yuqi, Qu, Xianjun, Yu, Xinfeng. 2024. PRDX1 inhibits ferroptosis by binding to Cullin-3 as a molecular chaperone in colorectal cancer. In International journal of biological sciences, 20, 5070-5086. doi:10.7150/ijbs.99804. https://pubmed.ncbi.nlm.nih.gov/39430237/
3. Li, Jing, Sun, Yuchen, Zhao, Xu, Sun, Xuanzi, Zhang, Xiaozhi. 2023. Radiation induces IRAK1 expression to promote radioresistance by suppressing autophagic cell death via decreasing the ubiquitination of PRDX1 in glioma cells. In Cell death & disease, 14, 259. doi:10.1038/s41419-023-05732-0. https://pubmed.ncbi.nlm.nih.gov/37031183/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen