C57BL/6JCya-Pax4em1/Cya
Common Name:
Pax4-KO
Product ID:
S-KO-03560
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Pax4-KO
Strain ID
KOCMP-18506-Pax4-B6J-VA
Gene Name
Product ID
S-KO-03560
Gene Alias
Pax-4
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pax4em1/Cya mice (Catalog S-KO-03560) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031718
NCBI RefSeq
NM_011038
Target Region
Exon 2~11
Size of Effective Region
~4.9 kb
Detailed Document
Overview of Gene Research
Pax4, short for Paired box 4, is a key transcription factor. It consists of a conserved paired box domain and a homeodomain, and is crucial in the embryonic development of the pancreatic islets of Langerhans. Pax4 plays an essential role in early endocrine progenitor cells, being necessary for cell-fate commitment towards the insulin-secreting β cell lineage [1,3,4].
In animal models, knockout of Pax4 leads to the absence of β cells, accompanied by a significant increase in glucagon-producing α cells, and typically results in lethality within days after birth [1,3]. In humans, mutations in Pax4 that cause impaired function are associated with diabetes pathogenesis. For example, the coding variant (p.Arg192His) in PAX4 is associated with an altered risk for type 2 diabetes (T2D) in East Asian populations. In a human beta cell model, PAX4 knockdown led to impaired insulin secretion, reduced total insulin content, and altered hormone gene expression [2]. Also, a mutation in the PAX4 gene (c.487C>T) can cause maturity-onset diabetes of the young (MODY9) [5].
In conclusion, Pax4 is essential for the development of insulin-secreting β cells. Gene knockout models in animals have clearly demonstrated its critical role in pancreatic islet development. In humans, Pax4 mutations are associated with diabetes, highlighting its importance in understanding diabetes pathogenesis and potentially developing new therapeutic strategies for diabetes treatment [1,2,3,4,5].
References:
1. Ko, Jenna, Fonseca, Vivian A, Wu, Hongju. 2023. Pax4 in Health and Diabetes. In International journal of molecular sciences, 24, . doi:10.3390/ijms24098283. https://pubmed.ncbi.nlm.nih.gov/37175989/
2. Lau, Hwee Hui, Krentz, Nicole A J, Abaitua, Fernando, Gloyn, Anna L, Teo, Adrian Kee Keong. 2023. PAX4 loss of function increases diabetes risk by altering human pancreatic endocrine cell development. In Nature communications, 14, 6119. doi:10.1038/s41467-023-41860-z. https://pubmed.ncbi.nlm.nih.gov/37777536/
3. Napolitano, Tiziana, Avolio, Fabio, Courtney, Monica, Navarro, Sergi, Collombat, Patrick. 2015. Pax4 acts as a key player in pancreas development and plasticity. In Seminars in cell & developmental biology, 44, 107-14. doi:10.1016/j.semcdb.2015.08.013. https://pubmed.ncbi.nlm.nih.gov/26319183/
4. Lorenzo, Petra I, Juárez-Vicente, Francisco, Cobo-Vuilleumier, Nadia, García-Domínguez, Mario, Gauthier, Benoit R. 2017. The Diabetes-Linked Transcription Factor PAX4: From Gene to Functional Consequences. In Genes, 8, . doi:10.3390/genes8030101. https://pubmed.ncbi.nlm.nih.gov/28282933/
5. Zhang, Di, Chen, Congli, Yang, Wenli, Ren, Li, Sang, Yanmei. . C.487C>T mutation in PAX4 gene causes MODY9: A case report and literature review. In Medicine, 101, e32461. doi:10.1097/MD.0000000000032461. https://pubmed.ncbi.nlm.nih.gov/36595822/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen