C57BL/6JCya-Pde4bem1/Cya
Common Name:
Pde4b-KO
Product ID:
S-KO-03592
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Pde4b-KO
Strain ID
KOCMP-18578-Pde4b-B6J-VA
Gene Name
Product ID
S-KO-03592
Gene Alias
Dpde4; dunce
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pde4bem1/Cya mice (Catalog S-KO-03592) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000106908
NCBI RefSeq
NM_019840
Target Region
Exon 8
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Pde4b, a member of the phosphodiesterase (PDEs) protein family, is a major cyclic adenosine monophosphate (cAMP)-metabolizing enzyme [3]. PDE4 enzymes hydrolyze intracellular cAMP, and Pde4b is one of its 4 subtypes (A-D). cAMP signaling is involved in numerous cellular processes, making Pde4b's function crucial in regulating these pathways [1].
In acute myocardial infarction, Pde4b deletion in mice strikingly reduced infarct size and improved cardiac function after myocardial ischemia-reperfusion (MI/R) injury. Pde4b in bone marrow-derived cells and vascular Pde4b promoted MI/R injury. Mechanistically, it mediated neutrophil-endothelial cell interaction, PKA-dependent expression of cell adhesion molecules, neutrophil cardiac infiltration, and release of proinflammatory cytokines. It also promoted coronary microcirculatory obstruction and vascular permeability [1]. In heart failure, cardiac overexpression of Pde4b in transgenic mouse lines had different effects. A moderate increase was cardioprotective, preventing systolic dysfunction, apoptosis, and fibrosis induced by chronic isoproterenol treatment or transverse aortic constriction, while a markedly higher overexpression led to hypertrophy, dilatation, and premature death [2].
In summary, Pde4b plays a significant role in regulating cAMP-related biological processes. Studies using gene knockout (KO) or conditional knockout (CKO) mouse models have revealed its involvement in acute myocardial infarction and heart failure. These models have been instrumental in understanding how Pde4b contributes to these disease conditions, highlighting its potential as a therapeutic target for treating cardiovascular diseases [1,2].
References:
1. Wan, Qing, Xu, Chuansheng, Zhu, Liyuan, Liu, De-Pei, Wang, Miao. 2022. Targeting PDE4B (Phosphodiesterase-4 Subtype B) for Cardioprotection in Acute Myocardial Infarction via Neutrophils and Microcirculation. In Circulation research, 131, 442-455. doi:10.1161/CIRCRESAHA.122.321365. https://pubmed.ncbi.nlm.nih.gov/35899614/
2. Karam, Sarah, Margaria, Jean Piero, Bourcier, Aurélia, Leroy, Jérôme, Vandecasteele, Grégoire. 2020. Cardiac Overexpression of PDE4B Blunts β-Adrenergic Response and Maladaptive Remodeling in Heart Failure. In Circulation, 142, 161-174. doi:10.1161/CIRCULATIONAHA.119.042573. https://pubmed.ncbi.nlm.nih.gov/32264695/
3. Su, Yue, Ding, Jiaxiang, Yang, Fan, Zhou, Huan, Li, Hongtao. 2022. The regulatory role of PDE4B in the progression of inflammatory function study. In Frontiers in pharmacology, 13, 982130. doi:10.3389/fphar.2022.982130. https://pubmed.ncbi.nlm.nih.gov/36278172/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen