C57BL/6NCya-Pfkfb1em1/Cya
Common Name:
Pfkfb1-KO
Product ID:
S-KO-03628
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Pfkfb1-KO
Strain ID
KOCMP-18639-Pfkfb1-B6N-VA
Gene Name
Product ID
S-KO-03628
Gene Alias
PFK/FBPase 1
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Pfkfb1em1/Cya mice (Catalog S-KO-03628) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000080884
NCBI RefSeq
NM_008824
Target Region
Exon 2~4
Size of Effective Region
~4.4 kb
Detailed Document
Overview of Gene Research
Pfkfb1, which encodes the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase-2), plays a crucial role in glucose homeostasis. This enzyme catalyzes the synthesis and degradation of fructose-2,6-bisphosphate, a key metabolite that allosterically regulates the rate-limiting enzyme 6-phosphofructo-1-kinase in the glycolytic pathway, thus controlling the overall rate of glycolysis [2,3,4,7,8].
In multiple myeloma, the STAT3-Pfkfb1 pathway is triggered downstream of LILRB4, which is highly expressed in multiple myeloma cell lines and patient samples. Knockdown of LILRB4 delays the growth of MM cells both in vitro and in vivo, suggesting that the STAT3-Pfkfb1 pathway is important for MM cell proliferation [1]. In sepsis-induced acute lung injury, esculetin inhibits the expression of glycolysis-related genes including Pfkfb1, rebalances M1/M2 macrophage polarization by regulating metabolic reprogramming, and improves the survival rate of rats [5]. In the context of rheumatoid arthritis, synovial fibroblasts under inflammatory conditions can upregulate the mRNA expression level of Pfkfb1 in synovial macrophages, inducing metabolic reprogramming and contributing to chronic inflammation [6].
In conclusion, Pfkfb1 is essential in regulating glycolysis and glucose metabolism. Studies related to Pfkfb1 have shed light on its roles in diseases such as multiple myeloma, sepsis-induced acute lung injury, and rheumatoid arthritis. Understanding the function of Pfkfb1 through these disease-related models can potentially provide new therapeutic strategies for these conditions.
References:
1. Xie, Li, Chen, Chiqi, Zhang, Tinghua, Yu, Zhuo, Zheng, Junke. 2024. LILRB4 regulates multiple myeloma development through STAT3-PFKFB1 pathway. In Cell death & disease, 15, 515. doi:10.1038/s41419-024-06883-4. https://pubmed.ncbi.nlm.nih.gov/39025844/
2. Cosin-Roger, Jesús, Vernia, Santiago, Alvarez, Maria Soledad, Fernández-Alvarez, Ana Julia, Casado, Marta. 2013. Identification of a novel Pfkfb1 mRNA variant in rat fetal liver. In Biochemical and biophysical research communications, 431, 36-40. doi:10.1016/j.bbrc.2012.12.109. https://pubmed.ncbi.nlm.nih.gov/23291237/
3. Bartrons, Ramon, Simon-Molas, Helga, Rodríguez-García, Ana, Manzano, Anna, Martinez-Outschoorn, Ubaldo E. 2018. Fructose 2,6-Bisphosphate in Cancer Cell Metabolism. In Frontiers in oncology, 8, 331. doi:10.3389/fonc.2018.00331. https://pubmed.ncbi.nlm.nih.gov/30234009/
4. Metón, Isidoro, Egea, Miriam, Anemaet, Ida G, Fernández, Felipe, Baanante, Isabel V. 2006. Sterol regulatory element binding protein-1a transactivates 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene promoter. In Endocrinology, 147, 3446-56. doi:. https://pubmed.ncbi.nlm.nih.gov/16614080/
5. Chen, Feng, Wang, Ning, Liao, Jiabao, Wen, Weibo, Chen, Fengjuan. . Esculetin rebalances M1/M2 macrophage polarization to treat sepsis-induced acute lung injury through regulating metabolic reprogramming. In Journal of cellular and molecular medicine, 28, e70178. doi:10.1111/jcmm.70178. https://pubmed.ncbi.nlm.nih.gov/39535339/
6. Saeki, Noritaka, Imai, Yuuki. 2020. Reprogramming of synovial macrophage metabolism by synovial fibroblasts under inflammatory conditions. In Cell communication and signaling : CCS, 18, 188. doi:10.1186/s12964-020-00678-8. https://pubmed.ncbi.nlm.nih.gov/33256735/
7. Chesney, Jason. . 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase and tumor cell glycolysis. In Current opinion in clinical nutrition and metabolic care, 9, 535-9. doi:. https://pubmed.ncbi.nlm.nih.gov/16912547/
8. Bartrons, Ramon, Caro, Jaime. . Hypoxia, glucose metabolism and the Warburg's effect. In Journal of bioenergetics and biomembranes, 39, 223-9. doi:. https://pubmed.ncbi.nlm.nih.gov/17661163/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen