C57BL/6JCya-Pik3caem1/Cya
Common Name
Pik3ca-KO
Product ID
S-KO-03651
Backgroud
C57BL/6JCya
Strain ID
KOCMP-18706-Pik3ca-B6J-VA
Status
When using this mouse strain in a publication, please cite “Pik3ca-KO Mouse (Catalog S-KO-03651) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Pik3ca-KO
Strain ID
KOCMP-18706-Pik3ca-B6J-VA
Gene Name
Product ID
S-KO-03651
Gene Alias
p110, caPI3K, p110alpha, 6330412C24Rik
Background
C57BL/6JCya
Gene Full Name
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
Modification
Conventional knockout
NCBI ID
Phenotype
Chromosome
Chr 3 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000108243
NCBI Transcript ID
NM_008839
Target Region
Exon 5~6
Size of Effective Region
~1.8 kb
Overview of Gene Research
PIK3CA, encoding the catalytic subunit p110α of Class IA Phosphatidylinositol 3-kinases (PI3K), is crucial in regulating cell survival, growth, cell cycle, and metabolism. It functions via the activation of the mTOR1-AKT pathway, a key signaling cascade involved in numerous biological processes [1,2].
Gain-of-function mutations in PIK3CA have been linked to a variety of disorders. Somatic activating mutations in PIK3CA are associated with vascular overgrowth syndromes, such as Klippel-Trenaunay syndrome, within the PIK3CA-related overgrowth spectrum (PROS). These mutations can lead to complications like thromboembolic events and orthopedic issues due to asymmetric overgrowth [1]. In cancer, PIK3CA mutations are prevalent, associated with resistance to first-line treatment and low survival rates in malignancies like colorectal cancer. There is evidence that PIK3CA-mutated colorectal cancer patients may benefit from aspirin treatment, though the exact mechanisms remain unclear [2]. PIK3CA mutations in solid malignancies are associated with poor prognosis, and inhibitors of the PI3K/AKT/mTOR pathway are being investigated as potential therapies [3]. In endometrial cancer, PIK3CA mutations seem to negatively influence survival outcomes, especially in low-grade tumors [4].
In conclusion, PIK3CA is essential in regulating key cellular functions through the mTOR1-AKT pathway. Research on PIK3CA-related disorders, especially those involving its mutations, has significant implications for understanding the pathogenesis of vascular overgrowth syndromes and various cancers. The study of PIK3CA provides potential therapeutic targets for these disease areas, highlighting the importance of further functional studies and in vivo research models such as KO/CKO mouse models to better elucidate its role in health and disease.
References:
1. Hughes, Meagan, Hao, Michelle, Luu, Minnelly. . PIK3CA vascular overgrowth syndromes: an update. In Current opinion in pediatrics, 32, 539-546. doi:10.1097/MOP.0000000000000923. https://pubmed.ncbi.nlm.nih.gov/32692051/
2. Hall, Daniella C N, Benndorf, Ralf A. 2022. Aspirin sensitivity of PIK3CA-mutated Colorectal Cancer: potential mechanisms revisited. In Cellular and molecular life sciences : CMLS, 79, 393. doi:10.1007/s00018-022-04430-y. https://pubmed.ncbi.nlm.nih.gov/35780223/
3. Willis, Owen, Choucair, Khalil, Alloghbi, Abdurahman, Dworkin, Lance, Nemunaitis, John. 2020. PIK3CA gene aberrancy and role in targeted therapy of solid malignancies. In Cancer gene therapy, 27, 634-644. doi:10.1038/s41417-020-0164-0. https://pubmed.ncbi.nlm.nih.gov/31988478/
4. Bredin, Hanna K, Krakstad, Camilla, Hoivik, Erling A. 2023. PIK3CA mutations and their impact on survival outcomes of patients with endometrial cancer: A systematic review and meta-analysis. In PloS one, 18, e0283203. doi:10.1371/journal.pone.0283203. https://pubmed.ncbi.nlm.nih.gov/36943861/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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