C57BL/6NCya-Ppp1r1bem1/Cya
Common Name
Ppp1r1b-KO
Product ID
S-KO-03761
Backgroud
C57BL/6NCya
Strain ID
KOCMP-19049-Ppp1r1b-B6N-VA
When using this mouse strain in a publication, please cite “Ppp1r1b-KO Mouse (Catalog S-KO-03761) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Ppp1r1b-KO
Strain ID
KOCMP-19049-Ppp1r1b-B6N-VA
Gene Name
Product ID
S-KO-03761
Gene Alias
DARPP-32, Darpp32
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 11
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000078694
NCBI RefSeq
NM_144828
Target Region
Exon 2~5
Size of Effective Region
~4.9 kb
Overview of Gene Research
Ppp1r1b, also known as Protein phosphatase 1 regulatory inhibitor subunit 1B, codes for multiple transcripts and two proteins, Darpp-32 and t-Darpp. Darpp-32 phosphorylation is upregulated by cAMP in dopamine-responsive brain tissues, modulating Protein Phosphatase 1 activity, which controls hundreds of phosphorylated proteins. PPP1R1B-knockout mice exhibit subtle learning defects [2].
In pancreatic cancer, loss of HIF1A increases PPP1R1B expression. EKPC mice with pancreas-specific deletion of HIF1A develop more advanced pancreatic neoplasias and PDACs with more invasion and metastasis. PPP1R1B promotes p53 degradation by stabilizing MDM2 phosphorylation at Ser166, and knockdown of PPP1R1B reduces the ability of pancreatic cancer cells to form lung metastases in mice [1]. In gastric cancer, the PPP1R1B-STARD3 chimeric fusion transcript, detected in 21.3% of primary human gastric cancers, promotes tumorigenesis through activation of PI3K/AKT signaling [3].
In summary, Ppp1r1b plays a crucial role in multiple biological processes and disease conditions. Gene-knockout mouse models have revealed its significance in cancer development, especially in pancreatic and gastric cancers, by influencing key cellular pathways related to tumor invasion, metastasis, and growth.
References:
1. Tiwari, Ashutosh, Tashiro, Kojiro, Dixit, Ajay, Provenzano, Paolo P, Bagchi, Anindya. 2020. Loss of HIF1A From Pancreatic Cancer Cells Increases Expression of PPP1R1B and Degradation of p53 to Promote Invasion and Metastasis. In Gastroenterology, 159, 1882-1897.e5. doi:10.1053/j.gastro.2020.07.046. https://pubmed.ncbi.nlm.nih.gov/32768595/
2. Avanes, Arabo, Lenz, Gal, Momand, Jamil. 2018. Darpp-32 and t-Darpp protein products of PPP1R1B: Old dogs with new tricks. In Biochemical pharmacology, 160, 71-79. doi:10.1016/j.bcp.2018.12.008. https://pubmed.ncbi.nlm.nih.gov/30552871/
3. Yun, S M, Yoon, K, Lee, S, Yang, H-K, Kim, S-J. 2013. PPP1R1B-STARD3 chimeric fusion transcript in human gastric cancer promotes tumorigenesis through activation of PI3K/AKT signaling. In Oncogene, 33, 5341-7. doi:10.1038/onc.2013.472. https://pubmed.ncbi.nlm.nih.gov/24276243/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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