C57BL/6JCya-Sardhem1/Cya
Common Name:
Sardh-KO
Product ID:
S-KO-03854
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Sardh-KO
Strain ID
KOCMP-192166-Sardh-B6J-VA
Gene Name
Product ID
S-KO-03854
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sardhem1/Cya mice (Catalog S-KO-03854) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000102886
NCBI RefSeq
NM_138665.2
Target Region
Exon 3~6
Size of Effective Region
~3.9 kb
Detailed Document
Overview of Gene Research
Sardh, or sarcosine dehydrogenase, is involved in the metabolism of the glycine-derivative sarcosine. It participates in the glycine production pathway, and is also linked to the purine cycle and one-carbon metabolism [1,4]. It has overall biological importance in processes like muscle function regulation, sex pheromone biosynthesis, and potentially in the immune system [1,3,2]. Genetic models can be valuable for studying its functions.
In Drosophila models, muscle-specific knockdown of Sardh leads to muscle dysfunction, ectopic lipid accumulation, and loss of the benefits of time-restricted feeding, suggesting its role in maintaining muscle function [1]. In male Bactrocera dorsalis, RNAi-mediated loss-of-function of Sardh decreases glycine, threonine (sex pheromone precursors), and sex pheromone contents, reducing male mating ability [3]. In children with sarcosinemia or in mice with Sardh deletion, formaldehyde deficiency due to the mutation of the mitochondrial Sardh gene leads to cognitive deficits, indicating its role in learning and memory [5].
In conclusion, Sardh plays essential roles in muscle function, sex pheromone biosynthesis, and learning and memory. Studies using gene knockout models in Drosophila, Bactrocera dorsalis, and mice have revealed its importance in these biological processes, providing insights into potential disease mechanisms related to muscle disorders, reproductive issues, and cognitive impairments.
References:
1. Livelo, Christopher, Guo, Yiming, Abou Daya, Farah, Panda, Satchidananda, Melkani, Girish C. 2023. Time-restricted feeding promotes muscle function through purine cycle and AMPK signaling in Drosophila obesity models. In Nature communications, 14, 949. doi:10.1038/s41467-023-36474-4. https://pubmed.ncbi.nlm.nih.gov/36810287/
2. Lakshmikanth, Tadepally, Consiglio, Camila, Sardh, Fabian, Landegren, Nils, Brodin, Petter. 2024. Immune system adaptation during gender-affirming testosterone treatment. In Nature, 633, 155-164. doi:10.1038/s41586-024-07789-z. https://pubmed.ncbi.nlm.nih.gov/39232147/
3. Gui, Shiyu, Yuval, Boaz, Engl, Tobias, Lu, Yongyue, Cheng, Daifeng. 2023. Protein feeding mediates sex pheromone biosynthesis in an insect. In eLife, 12, . doi:10.7554/eLife.83469. https://pubmed.ncbi.nlm.nih.gov/36656757/
4. Green, Thomas, Chen, Xiaofei, Ryan, Stephen, Asch, Adam S, Ruiz-Echevarría, Maria J. 2013. TMEFF2 and SARDH cooperate to modulate one-carbon metabolism and invasion of prostate cancer cells. In The Prostate, 73, 1561-75. doi:10.1002/pros.22706. https://pubmed.ncbi.nlm.nih.gov/23824605/
5. Ai, Li, Tan, Tao, Tang, Yonghe, Cai, Xiang, Tong, Zhiqian. 2019. Endogenous formaldehyde is a memory-related molecule in mice and humans. In Communications biology, 2, 446. doi:10.1038/s42003-019-0694-x. https://pubmed.ncbi.nlm.nih.gov/31815201/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen