C57BL/6JCya-Rab33bem1/Cya
Common Name:
Rab33b-KO
Product ID:
S-KO-03977
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Rab33b-KO
Strain ID
KOCMP-19338-Rab33b-B6J-VA
Gene Name
Product ID
S-KO-03977
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
3
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rab33bem1/Cya mice (Catalog S-KO-03977) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000054387
NCBI RefSeq
NM_016858.2
Target Region
Exon 1
Size of Effective Region
~4.8 kb
Detailed Document
Overview of Gene Research
Rab33b, a member of the RAS oncogene family, localizes to the Golgi apparatus. It functions in both membrane trafficking and autophagic processes, with a significant role in maintaining cellular homeostasis [4]. In membrane trafficking, it has been shown to regulate Golgi-to-ER retrograde trafficking, Golgi homeostasis, and post-Golgi transport to the plasma membrane [1]. In autophagy, it is involved in autophagosome formation [2,3].
A Rab33b missense mouse model for Smith-McCort dysplasia (SMC) showed bone resorption defects and altered protein glycosylation. The mutation in male mice led to changes in trabecular and cortical bone thickness, suggesting a role of Rab33b in osteoclast function [5]. RNA interference-based studies in hepatoma cell lines indicated that Rab33B is required for hepatitis B virus (HBV) propagation, guiding core transport to NC assembly and budding sites [6].
In conclusion, Rab33b is crucial for membrane trafficking and autophagy. The Rab33b missense mouse model has revealed its role in SMC-related bone resorption defects, and in vitro studies have shown its importance in HBV propagation. These findings contribute to understanding the molecular mechanisms of these biological processes and diseases related to Rab33b dysfunction.
References:
1. Bjørnestad, Synne Arstad, Guadagno, Noemi Antonella, Kjos, Ingrid, Progida, Cinzia. 2022. Rab33b-exocyst interaction mediates localized secretion for focal adhesion turnover and cell migration. In iScience, 25, 104250. doi:10.1016/j.isci.2022.104250. https://pubmed.ncbi.nlm.nih.gov/35521520/
2. Pantoom, Supansa, Konstantinidis, Georgios, Voss, Stephanie, Li, Zhiyu, Wu, Yao-Wen. 2020. RAB33B recruits the ATG16L1 complex to the phagophore via a noncanonical RAB binding protein. In Autophagy, 17, 2290-2304. doi:10.1080/15548627.2020.1822629. https://pubmed.ncbi.nlm.nih.gov/32960676/
3. Metje-Sprink, Janina, Groffmann, Johannes, Neumann, Piotr, Schalk, Amanda M, Binotti, Beyenech. 2020. Crystal structure of the Rab33B/Atg16L1 effector complex. In Scientific reports, 10, 12956. doi:10.1038/s41598-020-69637-0. https://pubmed.ncbi.nlm.nih.gov/32737358/
4. Morgan, Niamh E, Cutrona, Meritxell B, Simpson, Jeremy C. 2019. Multitasking Rab Proteins in Autophagy and Membrane Trafficking: A Focus on Rab33b. In International journal of molecular sciences, 20, . doi:10.3390/ijms20163916. https://pubmed.ncbi.nlm.nih.gov/31408960/
5. Dimori, Milena, Pokrovskaya, Irina D, Liu, Shijie, Lupashin, Vladimir V, Morello, Roy. 2023. A Rab33b missense mouse model for Smith-McCort dysplasia shows bone resorption defects and altered protein glycosylation. In Frontiers in genetics, 14, 1204296. doi:10.3389/fgene.2023.1204296. https://pubmed.ncbi.nlm.nih.gov/37359363/
6. Bartusch, Christina, Döring, Tatjana, Prange, Reinhild. 2017. Rab33B Controls Hepatitis B Virus Assembly by Regulating Core Membrane Association and Nucleocapsid Processing. In Viruses, 9, . doi:10.3390/v9060157. https://pubmed.ncbi.nlm.nih.gov/28635671/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen