C57BL/6JCya-Dcdc2aem1/Cya
Common Name:
Dcdc2a-KO
Product ID:
S-KO-04039
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dcdc2a-KO
Strain ID
KOCMP-195208-Dcdc2a-B6J-VA
Gene Name
Product ID
S-KO-04039
Gene Alias
AW492955; Dcdc2; RU2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dcdc2aem1/Cya mice (Catalog S-KO-04039) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000069614
NCBI RefSeq
NM_177577
Target Region
Exon 2
Size of Effective Region
~6.5 kb
Detailed Document
Overview of Gene Research
Dcdc2a, a member of the doublecortin domain-containing protein superfamily, likely plays a role in microtubule formation and stabilization, which is crucial for normal cellular functions related to cilia. It may be involved in pathways related to the development and function of inner ear cells [1].
A point mutation in Dcdc2a causes non-syndromic recessive deafness DFNB66 in a Tunisian family. In the rat inner ear, Dcdc2a localizes to the kinocilia of sensory hair cells and the primary cilia of non-sensory supporting cells. Overexpression in non-polarized COS7 cells induces long microtubule-based cytosolic cables. In hair and supporting cells, the deafness mutant Dcdc2a causes cilium structural defects and length ratio increases. In zebrafish, the ortholog dcdc2b is essential for hair cell development, survival, and function [1].
In conclusion, Dcdc2a is a key gene in regulating the length of hair cell kinocilia and supporting cell primary cilia, likely through its role in microtubule formation and stabilization. The findings from these genetic models, including zebrafish, help to understand its role in the context of deafness, revealing its importance in inner ear cell function and the development of this sensory deficit [1].
References:
1. Grati, M'hamed, Chakchouk, Imen, Ma, Qi, Liu, Xue Zhong, Masmoudi, Saber. 2015. A missense mutation in DCDC2 causes human recessive deafness DFNB66, likely by interfering with sensory hair cell and supporting cell cilia length regulation. In Human molecular genetics, 24, 2482-91. doi:10.1093/hmg/ddv009. https://pubmed.ncbi.nlm.nih.gov/25601850/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen