C57BL/6NCya-Ring1em1/Cya
Common Name:
Ring1-KO
Product ID:
S-KO-04099
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ring1-KO
Strain ID
KOCMP-19763-Ring1-B6N-VA
Gene Name
Product ID
S-KO-04099
Gene Alias
Ring1A
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Ring1em1/Cya mice (Catalog S-KO-04099) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025183
NCBI RefSeq
NM_009066
Target Region
Exon 3~5
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Ring1, also known as Ring finger protein 1, is a crucial component of the polycomb-group proteins. It is involved in multiple biological processes, such as chromatin modification as a catalytic subunit within Polycomb repressive complex 1 (PRC1), which catalyzes histone H2A lysine 119 monoubiquitination (H2AK119ub1) [1]. Ring1 has been associated with pathways like Wnt/β-catenin, and is important in processes like neurogenesis, germ-cell-specific transcription, and tumorigenesis [1,2,4]. Gene knockout models can be valuable for studying its functions.
In a neurodevelopment in vitro model, hypomorphic RING1 in PRC1 complexes leads to reduced H2AK119ub1, causing delayed DNA damage repair and cell cycle progression in neural progenitor cells (NPCs), while not significantly impacting NPC differentiation [1]. In pancreatic cancer, UBE2T-mediated ubiquitination of p53 by RING1 confers gemcitabine resistance [3]. In hepatic progenitor cells, overexpression of Ring1 promotes their transformation into cancer stem cells via the Wnt/β-catenin signaling pathway [4]. In tomato plants, the E3 ubiquitin ligase RING1 interacts with COP9 Signalosome Subunit 4 to positively regulate resistance to root-knot nematodes [5].
In conclusion, Ring1 plays essential roles in various biological processes, from neurogenesis to plant defense and tumorigenesis. Findings from gene-based research models, especially in the context of disease-related processes like cancer and in plant-pathogen interactions, highlight its significance. These studies help in understanding the underlying molecular mechanisms and potentially developing targeted interventions.
References:
1. Ryan, C W, Regan, S L, Mills, E F, Srivastava, A, Bielas, S L. 2024. RING1 missense variants reveal sensitivity of DNA damage repair to H2A monoubiquitination dosage during neurogenesis. In Nature communications, 15, 7931. doi:10.1038/s41467-024-52292-8. https://pubmed.ncbi.nlm.nih.gov/39256363/
2. Bajusz, Izabella, Henry, Surya, Sutus, Enikő, Kovács, Gergő, Pirity, Melinda K. 2019. Evolving Role of RING1 and YY1 Binding Protein in the Regulation of Germ-Cell-Specific Transcription. In Genes, 10, . doi:10.3390/genes10110941. https://pubmed.ncbi.nlm.nih.gov/31752312/
3. Jiang, Xiangyan, Ma, Yong, Wang, Tao, Yu, Zeyuan, Jiao, Zuoyi. 2023. Targeting UBE2T Potentiates Gemcitabine Efficacy in Pancreatic Cancer by Regulating Pyrimidine Metabolism and Replication Stress. In Gastroenterology, 164, 1232-1247. doi:10.1053/j.gastro.2023.02.025. https://pubmed.ncbi.nlm.nih.gov/36842710/
4. Zhu, Kai, Li, Jiangwei, Li, Jun, Kong, Guangyao, Li, Zongfang. 2019. Ring1 promotes the transformation of hepatic progenitor cells into cancer stem cells through the Wnt/β-catenin signaling pathway. In Journal of cellular biochemistry, 121, 3941-3951. doi:10.1002/jcb.29496. https://pubmed.ncbi.nlm.nih.gov/31696964/
5. Zou, Jin-Ping, Zhao, Qiu-Feng, Yang, Ting, Ahammed, Golam Jalal, Zhou, Jie. 2022. The E3 ubiquitin ligase RING1 interacts with COP9 Signalosome Subunit 4 to positively regulate resistance to root-knot nematodes in Solanum lycopersicum L. In Plant science : an international journal of experimental plant biology, 322, 111344. doi:10.1016/j.plantsci.2022.111344. https://pubmed.ncbi.nlm.nih.gov/35659944/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen