Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6NCya-Rps6ka2em1/Cya
Common Name:
Rps6ka2-KO
Product ID:
S-KO-04156
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Rps6ka2-KO
Strain ID
KOCMP-20112-Rps6ka2-B6N-VA
Gene Name
Rps6ka2
Product ID
S-KO-04156
Gene Alias
90kDa; D17Wsu134e; Rps6ka-rs1; Rsk3; p90rsk; pp90rsk
Background
C57BL/6NCya
NCBI ID
20112
Modification
Conventional knockout
Chromosome
17
Phenotype
MGI:1342290
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Rps6ka2em1/Cya mice (Catalog S-KO-04156) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000024575
NCBI RefSeq
NM_011299
Target Region
Exon 4~6
Size of Effective Region
~4.9 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Rps6ka2, also known as p90 ribosomal S6 kinase-3 (p90 Rsk-3), is a serine-threonine kinase that signals downstream of the mitogen-activated protein kinase pathway [3]. It plays a role in various biological processes and is associated with multiple diseases.

In a mouse model of knee osteoarthritis (OA), Nuclease technology-mediated Rps6ka2 knockout in iMSC (induced mesenchymal stem cells) showed that Rps6ka2 could promote iMSC proliferation and chondrogenic differentiation in vitro. In vivo, it improved iMSC viability, promoted extracellular matrix (ECM) production, and attenuated OA [1].

In ovarian cancer, bioinformatic analysis identified Rps6ka2 as a prognosis-related gene. Its expression was down-regulated in ovarian cancer tissues. Overexpression of Rps6ka2 suppressed cell proliferation, while knockdown had the opposite effect, and it was regulated by miRNAs and circRNAs and affected the p38/MAPK signaling pathway [2].

In sporadic epithelial ovarian cancer, Rps6ka2 was considered a putative tumour suppressor gene. Homozygous deletions were found in some cell lines, and re-expression of Rps6ka2 in ovarian cancer cell lines suppressed colony formation, reduced proliferation, caused G1 arrest, and increased apoptosis [3].

In colorectal cancer, Rps6ka2 inhibited the proliferation, migration, and invasion of CRC cells by interacting with PCSK9 and suppressing the PCSK9/MAPK signaling pathway [4].

In pancreatic cancer, a kinome-wide siRNA-based loss-of-function screen identified Rps6ka2 as a modifier of epidermal growth factor receptor (EGFR) activity. Inhibition of Rps6ka2 synergistically enhanced the effect of the EGFR inhibitor erlotinib on tumor cell survival [5].

In conclusion, Rps6ka2 plays important roles in multiple disease conditions such as knee osteoarthritis, ovarian cancer, sporadic epithelial ovarian cancer, colorectal cancer, and pancreatic cancer. Gene knockout models in mice or loss-of-function experiments in cell lines have been crucial in revealing its functions in promoting cell proliferation, chondrogenic differentiation, and its roles in tumor suppression or growth regulation through various signaling pathways. These findings contribute to understanding the biological mechanisms of these diseases and may provide potential therapeutic targets.

References:

1. Zhang, Juan, Liao, Jin-Qi, Wen, Li-Ru, Yang, Jian-Hua, Zhou, Guang-Qian. 2023. Rps6ka2 enhances iMSC chondrogenic differentiation to attenuate knee osteoarthritis through articular cartilage regeneration in mice. In Biochemical and biophysical research communications, 663, 61-70. doi:10.1016/j.bbrc.2023.04.049. https://pubmed.ncbi.nlm.nih.gov/37119767/

2. Fu, Zhiqin, Ding, Chao, Gong, Wangang, Lu, Chao. 2023. ncRNAs mediated RPS6KA2 inhibits ovarian cancer proliferation via p38/MAPK signaling pathway. In Frontiers in oncology, 13, 1028301. doi:10.3389/fonc.2023.1028301. https://pubmed.ncbi.nlm.nih.gov/36741009/

3. Bignone, P A, Lee, K Y, Liu, Y, Mungall, A J, Ganesan, T S. 2006. RPS6KA2, a putative tumour suppressor gene at 6q27 in sporadic epithelial ovarian cancer. In Oncogene, 26, 683-700. doi:. https://pubmed.ncbi.nlm.nih.gov/16878154/

4. Wang, Yu, Wang, Yuting, Gao, Huabin, Shi, Huijuan, Han, Anjia. 2025. Ezetimibe mediated RPS6KA2 inhibits colorectal cancer proliferation via PCSK9/MAPK signaling pathway. In Cancer treatment and research communications, 43, 100899. doi:10.1016/j.ctarc.2025.100899. https://pubmed.ncbi.nlm.nih.gov/40112524/

5. Milosevic, Nada, Kühnemuth, Benjamin, Mühlberg, Leonie, Gress, Thomas, Michl, Patrick. . Synthetic lethality screen identifies RPS6KA2 as modifier of epidermal growth factor receptor activity in pancreatic cancer. In Neoplasia (New York, N.Y.), 15, 1354-62. doi:. https://pubmed.ncbi.nlm.nih.gov/24403857/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest