C57BL/6JCya-Slc3a1em1/Cya
Common Name:
Slc3a1-KO
Product ID:
S-KO-04371
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Slc3a1-KO
Strain ID
KOCMP-20532-Slc3a1-B6J-VA
Gene Name
Product ID
S-KO-04371
Gene Alias
D2; D2H; NBAT; NTAA; RBAT
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc3a1em1/Cya mice (Catalog S-KO-04371) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000024944
NCBI RefSeq
NM_009205
Target Region
Exon 2~4
Size of Effective Region
~4.9 kb
Detailed Document
Overview of Gene Research
Slc3a1, also known as solute carrier family 3 (cystine, basic and) neutral amino acid transporter, member 1, codes for the protein related to the system bo,+ amino-acid transporter (rBAT) [3]. It is a crucial component of the b0,+ transporter system, which is involved in the reabsorption of cystine, ornithine, lysine, and arginine in the renal proximal tubule [2,3,4,6]. Mutations in Slc3a1 are associated with cystinuria, a genetic disorder leading to high concentrations of cystine in the urine and recurrent nephrolithiasis [2,3,4,5,6,7]. Genetic models, such as knockout mice, are valuable for studying its functions.
In Slc3a1 knockout male mice, more severe stone formation and renal injuries were observed compared to females, suggesting its role in the sexual dimorphism of cystinuria [1]. Further investigations revealed that higher SLC3A1 levels in male kidneys enhance mitochondrial functions through modulation of mitochondrial NAD+ uptake, primarily in proximal tubule cells. This aberrant mitochondrial function was the primary factor contributing to the severity of cystinuria in Slc3a1 knockout male mice [1].
In conclusion, Slc3a1 is essential for the normal reabsorption of certain amino acids in the renal proximal tubule. Its deficiency in male mice models reveals its role in the sexual dimorphism of cystinuria, mainly through its impact on mitochondrial functions. These findings from Slc3a1 knockout mouse models provide novel insights into the potential interventions for cystinuria [1].
References:
1. Su, Jingyi, Pan, Yongdong, Zhong, Fengbo, Guan, Yuting, Xu, Guofeng. 2024. Mitochondrial SLC3A1 regulates sexual dimorphism in cystinuria. In Genes & diseases, 12, 101472. doi:10.1016/j.gendis.2024.101472. https://pubmed.ncbi.nlm.nih.gov/40110490/
2. D'Ambrosio, Viola, Capolongo, Giovanna, Goldfarb, David, Gambaro, Giovanni, Ferraro, Pietro Manuel. 2021. Cystinuria: an update on pathophysiology, genetics, and clinical management. In Pediatric nephrology (Berlin, Germany), 37, 1705-1711. doi:10.1007/s00467-021-05342-y. https://pubmed.ncbi.nlm.nih.gov/34812923/
3. Breuning, M H, Hamdy, N A T C. . [From gene to disease; SLC3A1, SLC7A9 and cystinuria]. In Nederlands tijdschrift voor geneeskunde, 147, 245-7. doi:. https://pubmed.ncbi.nlm.nih.gov/12621979/
4. Mattoo, Aditya, Goldfarb, David S. . Cystinuria. In Seminars in nephrology, 28, 181-91. doi:10.1016/j.semnephrol.2008.01.011. https://pubmed.ncbi.nlm.nih.gov/18359399/
5. Liu, Danhua, Zhao, Yongli, Xue, Xia, Guo, Jiancheng, Xu, Changbao. 2023. Novel compound heterozygous pathogenic variants in the SLC3A1 gene in a Chinese family with cystinuria. In BMC medical genomics, 16, 333. doi:10.1186/s12920-023-01767-6. https://pubmed.ncbi.nlm.nih.gov/38114997/
6. Dello Strologo, Luca, Rizzoni, Gianfranco. . Cystinuria. In Acta paediatrica (Oslo, Norway : 1992). Supplement, 95, 31-3. doi:. https://pubmed.ncbi.nlm.nih.gov/16801163/
7. Bai, Peide, Zhang, WenZhao, Lai, Longhui, Wang, Tao, Chen, Bin. 2023. Declaration: Novel SLC3A1 mutation in a cystinuria patient with xanthine stones: a case report. In BMC urology, 23, 130. doi:10.1186/s12894-023-01300-y. https://pubmed.ncbi.nlm.nih.gov/37525149/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen