C57BL/6JCya-Snai1em1/Cya
Common Name
Snai1-KO
Product ID
S-KO-04397
Backgroud
C57BL/6JCya
Strain ID
KOCMP-20613-Snai1-B6J-VA
Status
When using this mouse strain in a publication, please cite “Snai1-KO Mouse (Catalog S-KO-04397) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Snai1-KO
Strain ID
KOCMP-20613-Snai1-B6J-VA
Gene Name
Product ID
S-KO-04397
Gene Alias
Sna, Sna1, Snail, Snail1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
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Strain Description
Ensembl Number
ENSMUST00000052631
NCBI RefSeq
NM_011427
Target Region
Exon 1~2
Size of Effective Region
~1.8 kb
Overview of Gene Research
Snai1, a zinc finger transcription factor, is a master regulator of epithelial-mesenchymal transition (EMT), which is crucial in various biological processes like embryonic development and cancer progression. It is also involved in immune regulation, stem cell properties, and metabolic regulation [2].
In a mouse model, hepatocyte-specific deletion of both Snai1 and Snai2, but not one alone, suppresses liver cyclin A2/D1 expression and regenerative hepatocyte proliferation after hepatectomy or CCl4 treatments, while augmenting CCl4-stimulated HSC activation and liver fibrosis. This indicates that hepatic Snai1 and Snai2 directly promote, via histone modifications, reparative hepatocyte replication and indirectly inhibit liver fibrosis [1]. In triple-negative breast cancer cells, knockout of SNAI1 by CRISPR/Cas9 uncovered an epithelio-mesenchymal phenotype accompanied by reduced TGFβ signaling, and the cells gained stemness potential and differentiation plasticity, drifting towards the luminal phenotype [3].
In conclusion, Snai1 is essential in EMT and other biological processes. The gene knockout models in mice have revealed its significance in liver regeneration, fibrosis, and breast cancer cell plasticity. Understanding Snai1's functions provides insights into the mechanisms of disease occurrence and may offer potential therapeutic strategies for liver diseases and breast cancer [1,3].
References:
1. Wang, Pingping, Kang, Qianqian, Wu, Wen-Shu, Rui, Liangyou. 2024. Hepatic Snai1 and Snai2 promote liver regeneration and suppress liver fibrosis in mice. In Cell reports, 43, 113875. doi:10.1016/j.celrep.2024.113875. https://pubmed.ncbi.nlm.nih.gov/38451818/
2. Dong, Bo, Wu, Yadi. 2021. Epigenetic Regulation and Post-Translational Modifications of SNAI1 in Cancer Metastasis. In International journal of molecular sciences, 22, . doi:10.3390/ijms222011062. https://pubmed.ncbi.nlm.nih.gov/34681726/
3. Tsirigoti, Chrysoula, Ali, Mohamad Moustafa, Maturi, Varun, Heldin, Carl-Henrik, Moustakas, Aristidis. 2022. Loss of SNAI1 induces cellular plasticity in invasive triple-negative breast cancer cells. In Cell death & disease, 13, 832. doi:10.1038/s41419-022-05280-z. https://pubmed.ncbi.nlm.nih.gov/36171192/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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