C57BL/6NCya-Sparcem1/Cya
Common Name
Sparc-KO
Product ID
S-KO-04435
Backgroud
C57BL/6NCya
Strain ID
KOCMP-20692-Sparc-B6N-VA
When using this mouse strain in a publication, please cite “Sparc-KO Mouse (Catalog S-KO-04435) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Sparc-KO
Strain ID
KOCMP-20692-Sparc-B6N-VA
Gene Name
Product ID
S-KO-04435
Gene Alias
ON, BM-40
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 11
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000018737
NCBI RefSeq
NM_009242
Target Region
Exon 3~8
Size of Effective Region
~10.9 kb
Overview of Gene Research
SPARC, also known as secreted protein acidic and rich in cysteine, osteonectin, or BM-40, is a matricellular protein. It regulates cell adhesion, extracellular matrix production, growth factor activity, and the cell cycle. It modulates cell-extracellular matrix interactions due to its anti-proliferative and anti-adhesion properties. SPARC is involved in multiple pathways, such as TGF-β1 or HIF2A signaling in pulmonary arterial smooth muscle cells (PASMCs), and TLR4-mediated TBK1, IRF3, IFN-β, and STAT1 signaling in macrophages [1,2]. It is important in various biological processes and diseases, including pulmonary hypertension, aging-related inflammation, and cancer [1-3].
In the context of pulmonary hypertension, adeno-associated virus-mediated Sparc knockdown in adult mice significantly improved hemodynamic and cardiac function in PH mice, suggesting its role in the pathogenesis of human PH and chronic hypoxia-induced PH in mice, likely by affecting vascular cell function [1]. In macrophages during aging, adipocyte-specific deletion of SPARC reduced inflammation and extended health span, indicating that SPARC is an immunometabolic checkpoint of inflammation and interferon response [2].
In conclusion, SPARC plays essential roles in multiple biological processes and diseases. The gene knockout or knockdown models in mice have been crucial in revealing its role in pulmonary hypertension and aging-related inflammation, providing insights into disease mechanisms and potential therapeutic targets.
References:
1. Veith, Christine, Vartürk-Özcan, Ipek, Wujak, Magdalena, Sommer, Natascha, Weissmann, Norbert. 2022. SPARC, a Novel Regulator of Vascular Cell Function in Pulmonary Hypertension. In Circulation, 145, 916-933. doi:10.1161/CIRCULATIONAHA.121.057001. https://pubmed.ncbi.nlm.nih.gov/35175782/
2. Ryu, Seungjin, Sidorov, Sviatoslav, Ravussin, Eric, Wang, Andrew, Dixit, Vishwa Deep. 2022. The matricellular protein SPARC induces inflammatory interferon-response in macrophages during aging. In Immunity, 55, 1609-1626.e7. doi:10.1016/j.immuni.2022.07.007. https://pubmed.ncbi.nlm.nih.gov/35963236/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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