C57BL/6JCya-Enpp3em1/Cya
Common Name
Enpp3-KO
Product ID
S-KO-04682
Backgroud
C57BL/6JCya
Strain ID
KOCMP-209558-Enpp3-B6J-VA
When using this mouse strain in a publication, please cite “Enpp3-KO Mouse (Catalog S-KO-04682) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Enpp3-KO
Strain ID
KOCMP-209558-Enpp3-B6J-VA
Gene Name
Product ID
S-KO-04682
Gene Alias
CD203c
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 10
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000020169
NCBI RefSeq
NM_134005
Target Region
Exon 2~4
Size of Effective Region
~8.5 kb
Overview of Gene Research
Enpp3, also known as ectonucleotide pyrophosphatase/phosphodiesterase 3, is an extracellular membrane protein. It belongs to the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family, which is involved in nucleotide and phospholipid signaling, among other biological processes [2]. Enpp3 has been identified as a major cGAMP hydrolase, regulating cGAMP levels to dampen anti-cancer immunity, and it also plays a role in innate immune cell-cell communication through extracellular cGAMP-STING signaling [1,3].
Selectively abolishing Enpp3's cGAMP hydrolysis activity in certain mouse models leads to diminished cancer growth and metastasis of some tumor types in a stimulator of interferon genes (STING)-dependent manner, indicating its role as an innate immune checkpoint [1,3]. In ENPP1-deficient mice, Enpp3 accounts for all cGAMP hydrolysis activity, highlighting its significance in this context [1,3].
In conclusion, Enpp3 is a crucial regulator in innate immune signaling, especially in controlling cGAMP-mediated anti-cancer immunity. Studies using gene-knockout mouse models have revealed its potential as a target for cancer immunotherapy, emphasizing the importance of this gene in cancer-related disease areas [1,3].
References:
1. Mardjuki, Rachel, Wang, Songnan, Carozza, Jacqueline, Goodarzi, Hani, Li, Lingyin. 2024. Identification of the extracellular membrane protein ENPP3 as a major cGAMP hydrolase and innate immune checkpoint. In Cell reports, 43, 114209. doi:10.1016/j.celrep.2024.114209. https://pubmed.ncbi.nlm.nih.gov/38749434/
2. Borza, Razvan, Salgado-Polo, Fernando, Moolenaar, Wouter H, Perrakis, Anastassis. 2021. Structure and function of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family: Tidying up diversity. In The Journal of biological chemistry, 298, 101526. doi:10.1016/j.jbc.2021.101526. https://pubmed.ncbi.nlm.nih.gov/34958798/
3. Mardjuki, Rachel, Wang, Songnan, Carozza, Jacqueline A, Lyu, Xuchao, Li, Lingyin. 2024. Identification of extracellular membrane protein ENPP3 as a major cGAMP hydrolase, cementing cGAMP's role as an immunotransmitter. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.01.12.575449. https://pubmed.ncbi.nlm.nih.gov/38260585/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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