C57BL/6NCya-Tent4aem1/Cya
Common Name
Tent4a-KO
Product ID
S-KO-04724
Backgroud
C57BL/6NCya
Strain ID
KOCMP-210106-Tent4a-B6N-VA
When using this mouse strain in a publication, please cite “Tent4a-KO Mouse (Catalog S-KO-04724) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Tent4a-KO
Strain ID
KOCMP-210106-Tent4a-B6N-VA
Gene Name
Product ID
S-KO-04724
Gene Alias
POLK, Pols, TRF4, LAK-1, Papd7, TRF4-1
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 13
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000198607
NCBI RefSeq
NM_198600
Target Region
Exon 4~6
Size of Effective Region
~3.4 kb
Overview of Gene Research
TENT4A, also known as PAPD7, is a non-canonical poly(A) polymerase. It plays a crucial role in regulating multiple biological pathways. It is involved in processes like translesion DNA synthesis (TLS), mRNA stability and translation, and RNA tailing, which are essential for maintaining genomic integrity and proper gene expression [1,2].
In DNA-damage tolerance, TENT4A knockdown leads to reduced amounts of RAD18 protein and DNA polymerase η, resulting in decreased TLS. This indicates its importance in this DNA-repair-related pathway. Bioinformatics analysis also shows that genes related to TLS error-prone DNA polymerases and their TENT4A-related regulators are frequently mutated in endometrial cancer genomes, suggesting a dysregulation of TLS in this cancer [1]. Additionally, depletion of TENT4A and its homolog TENT4B leads to a decrease in mRNA half-life and abundance in cells, highlighting their role in shielding mRNA from rapid deadenylation through generating a mixed poly(A) tail [2].
In summary, TENT4A is essential for regulating mRNA stability and translation, as well as DNA-damage tolerance pathways. The study of TENT4A through gene knockdown models has revealed its significance in endometrial cancer-related DNA-repair dysregulation and mRNA regulation, providing insights into potential disease mechanisms and therapeutic targets.
References:
1. Swain, Umakanta, Friedlander, Gilgi, Sehrawat, Urmila, Geacintov, Nicholas E, Livneh, Zvi. 2021. TENT4A Non-Canonical Poly(A) Polymerase Regulates DNA-Damage Tolerance via Multiple Pathways That Are Mutated in Endometrial Cancer. In International journal of molecular sciences, 22, . doi:10.3390/ijms22136957. https://pubmed.ncbi.nlm.nih.gov/34203408/
2. Lim, Jaechul, Kim, Dongwan, Lee, Young-Suk, Ahn, Kwangseog, Kim, V Narry. 2018. Mixed tailing by TENT4A and TENT4B shields mRNA from rapid deadenylation. In Science (New York, N.Y.), 361, 701-704. doi:10.1126/science.aam5794. https://pubmed.ncbi.nlm.nih.gov/30026317/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.