C57BL/6NCya-Ccdc157em1/Cya
Common Name:
Ccdc157-KO
Product ID:
S-KO-05178
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ccdc157-KO
Strain ID
KOCMP-216516-Ccdc157-B6N-VA
Gene Name
Product ID
S-KO-05178
Gene Alias
4930562D19Rik; mKIAA1656
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Ccdc157em1/Cya mice (Catalog S-KO-05178) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000093381
NCBI RefSeq
NM_001164620
Target Region
Exon 4~9
Size of Effective Region
~3.9 kb
Detailed Document
Overview of Gene Research
CCDC157, a chromosomal protein, is involved in multiple biological processes. It plays a crucial role in sperm differentiation, and is also associated with protein transport and secretion as it is an important factor for the fusion of transport carriers to Golgi membranes [3]. In addition, it has been linked to the regulation of genes involved in cell migration/motility and Golgi components [1]. Genetic models, such as mouse models, have been valuable in studying its functions.
In a mouse model, loss of Ccdc157 led to male sterility with oligoasthenoteratozoospermia (OAT)-like phenotypes. Electron microscopy revealed misstructured acrosome and abnormal head-tail coupling apparatus in the sperm of Ccdc157-null mice. Comparative transcriptome analysis showed that the Ccdc157 mutation alters the expressions of genes involved in cell migration/motility and Golgi components. Abnormal Golgi apparatus and decreased expressions of genes involved in acrosome formation and lipid metabolism were detected in Ccdc157-deprived mouse germ cells [1]. In Drosophila, the homolog of CCDC157, Pif1A, is essential for spermatide individualization and involved in the regulation of lipid metabolism genes [2].
In conclusion, CCDC157 is essential for sperm maturation as demonstrated through mouse and Drosophila models. The gene knockout studies in mice have revealed its significance in male fertility, specifically in relation to OAT. These findings suggest CCDC157 may serve as a diagnostic marker for OAT and provide insights into the mechanisms underlying male infertility [1].
References:
1. Zheng, Huimei, Gong, Chenjia, Li, Jingping, Yang, Xiaohang, Xi, Yongmei. . CCDC157 is essential for sperm differentiation and shows oligoasthenoteratozoospermia-related mutations in men. In Journal of cellular and molecular medicine, 28, e18215. doi:10.1111/jcmm.18215. https://pubmed.ncbi.nlm.nih.gov/38509755/
2. Yuan, Xin, Zheng, Huimei, Su, Yang, Xi, Yongmei, Yang, Xiaohang. 2019. Drosophila Pif1A is essential for spermatogenesis and is the homolog of human CCDC157, a gene associated with idiopathic NOA. In Cell death & disease, 10, 125. doi:10.1038/s41419-019-1398-3. https://pubmed.ncbi.nlm.nih.gov/30741974/
3. Bassaganyas, Laia, Popa, Stephanie J, Horlbeck, Max, Moreau, Kevin, Villeneuve, Julien. 2019. New factors for protein transport identified by a genome-wide CRISPRi screen in mammalian cells. In The Journal of cell biology, 218, 3861-3879. doi:10.1083/jcb.201902028. https://pubmed.ncbi.nlm.nih.gov/31488582/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen