C57BL/6JCya-Nlgn2em1/Cya
Common Name
Nlgn2-KO
Product ID
S-KO-05226
Backgroud
C57BL/6JCya
Strain ID
KOCMP-216856-Nlgn2-B6J-VA
When using this mouse strain in a publication, please cite “Nlgn2-KO Mouse (Catalog S-KO-05226) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Nlgn2-KO
Strain ID
KOCMP-216856-Nlgn2-B6J-VA
Gene Name
Product ID
S-KO-05226
Gene Alias
NL2, NLG2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 11
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000056484
NCBI RefSeq
NM_198862
Target Region
Exon 1~7
Size of Effective Region
~8.4 kb
Overview of Gene Research
Nlgn2, or Neuroligin-2, is a key synaptic adhesion protein predominantly found at GABAergic synapses. It is crucial for recruiting GABAARs by facilitating the assembly of the postsynaptic gephyrin scaffold, thus playing a vital role in inhibitory synaptic transmission [1]. Genetic models, such as KO mouse models, are valuable for studying Nlgn2's functions.
In Nlgn2 knockout mice, there are abnormal cytosolic gephyrin aggregation, reduced inhibitory synaptic transmission, and exacerbated anxiety-related behavior [1]. In schizophrenia, iPSC-derived cortical interneurons from patients show reduced levels of NLGN2, along with synaptic deficits. Overexpression of NLGN2 in these neurons rescues synaptic puncta deficits, while knockdown in healthy neurons leads to reduced synaptic puncta density [2]. In the context of pancreatic intraepithelial neoplasia, NLGN2 expressed by pancreatic exocrine cells is involved in regulating contact inhibition and epithelial polarity, with its loss associated with the progression from low-to high-grade lesions [3].
In summary, Nlgn2 is essential for proper inhibitory synaptic function at GABAergic synapses. Studies using gene knockout models have revealed its significant roles in mental health-related conditions like schizophrenia and anxiety, as well as in pancreatic tumor progression. Understanding Nlgn2 through these models provides insights into the underlying mechanisms of these diseases, potentially guiding the development of new therapeutic strategies.
References:
1. Zeppillo, Tommaso, Ali, Heba, Ravichandran, Sowbarnika, Taschenberger, Holger, Krueger-Burg, Dilja. 2024. Functional Neuroligin-2-MDGA1 interactions differentially regulate synaptic GABAARs and cytosolic gephyrin aggregation. In Communications biology, 7, 1157. doi:10.1038/s42003-024-06789-z. https://pubmed.ncbi.nlm.nih.gov/39284869/
2. Kathuria, Annie, Lopez-Lengowski, Kara, Watmuff, Bradley, Cohen, Bruce M, Karmacharya, Rakesh. 2019. Synaptic deficits in iPSC-derived cortical interneurons in schizophrenia are mediated by NLGN2 and rescued by N-acetylcysteine. In Translational psychiatry, 9, 321. doi:10.1038/s41398-019-0660-x. https://pubmed.ncbi.nlm.nih.gov/31780643/
3. Middonti, Emanuele, Astanina, Elena, Vallariello, Edoardo, Arese, Marco, Bussolino, Federico. 2024. A neuroligin-2-YAP axis regulates progression of pancreatic intraepithelial neoplasia. In EMBO reports, 25, 1886-1908. doi:10.1038/s44319-024-00104-x. https://pubmed.ncbi.nlm.nih.gov/38413734/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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