C57BL/6JCya-Proca1em1/Cya
Common Name
Proca1-KO
Product ID
S-KO-05239
Backgroud
C57BL/6JCya
Strain ID
KOCMP-216974-Proca1-B6J-VA
When using this mouse strain in a publication, please cite “Proca1-KO Mouse (Catalog S-KO-05239) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Proca1-KO
Strain ID
KOCMP-216974-Proca1-B6J-VA
Gene Name
Product ID
S-KO-05239
Gene Alias
4933404M19Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 11
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000078099
NCBI RefSeq
NM_001045516
Target Region
Exon 3~4
Size of Effective Region
~1.2 kb
Overview of Gene Research
Proca1, also known as protein interacting with cyclin A1, has diverse functions. It has been implicated in various biological processes including fatty acid metabolism-related pathways, which may impact anti-PD1 therapy efficacy in oral tongue squamous cell carcinoma [1]. It is also potentially involved in male infertility, with its expression in the testis, specifically localized to the acrosome of normal human sperm [2,3].
In male infertility research, three patients with acrosome-deficient teratozoospermia were found to have PROCA1 mutations by whole exome sequencing. However, Proca1 knockout (KO) mice generated using CRISPR-Cas9 technology were fertile, with no significant differences in testicular tissue, sperm morphology, or acrosome marker expression compared to wild-type mice. This suggests that the PROCA1 mutation identified in humans may not affect acrosome biogenesis in mice [3].
In conclusion, Proca1 plays potential roles in fatty acid metabolism-related immunotherapy responses and male infertility. The Proca1 KO mouse model has been valuable in revealing that the human PROCA1 mutation may not impact acrosome formation in mice, contributing to our understanding of its function in male infertility.
References:
1. Tan, Xiyan, Li, Guoli, Deng, Honghao, Zhang, Chenzhi, Chen, Yanfeng. . Obesity enhances the response to neoadjuvant anti-PD1 therapy in oral tongue squamous cell carcinoma. In Cancer medicine, 13, e7346. doi:10.1002/cam4.7346. https://pubmed.ncbi.nlm.nih.gov/38923758/
2. Omolaoye, Temidayo S, Omolaoye, Victor A, Kandasamy, Richard K, Hachim, Mahmood Yaseen, Du Plessis, Stefan S. 2022. Omics and Male Infertility: Highlighting the Application of Transcriptomic Data. In Life (Basel, Switzerland), 12, . doi:10.3390/life12020280. https://pubmed.ncbi.nlm.nih.gov/35207567/
3. Zheng, Na, Shen, Yiru, Wang, Yu, Zhu, Fuxi, Cao, Yunxia. 2024. Unraveling the Impact of the PROCA1 Mutation in Male Infertility: Incorporating Whole Exome Sequencing in Teratozoospermia Patients and Analyzing Proca1 Knockout Mice. In Reproductive sciences (Thousand Oaks, Calif.), 32, 1080-1091. doi:10.1007/s43032-024-01624-6. https://pubmed.ncbi.nlm.nih.gov/38867036/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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