C57BL/6JCya-Tgfb1em1/Cya
Common Name:
Tgfb1-KO
Product ID:
S-KO-05368
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Tgfb1-KO
Strain ID
KOCMP-21803-Tgfb1-B6J-VA
Gene Name
Product ID
S-KO-05368
Gene Alias
TGF-beta1; TGFbeta1; Tgfb; Tgfb-1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tgfb1em1/Cya mice (Catalog S-KO-05368) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000002678
NCBI RefSeq
NM_011577
Target Region
Exon 2
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Tgfb1, encoding TGF-β1, is a gene for a potent cytokine that regulates diverse cellular processes such as immune responses, cell growth, differentiation, and apoptosis. It is involved in multiple signaling pathways including the SMAD-dependent pathway, which is crucial for its downstream effects. TGF-β1 is of great biological importance in tissue repair, development, and disease processes [3].
In the context of intestinal regeneration, irradiation-induced damage leads to a surge in Tgfb1 expression mediated by monocyte/macrophage cells at the damage site. Depletion of macrophages or genetic disruption of TGF-β signaling significantly impairs the regenerative response. Tgfb1 treatment in organoid culture can induce a fetal-like/regenerative state, and it also activates pro-regenerative factors YAP/TEAD and SOX9 in the epithelium, suggesting its key role in enhancing intestinal regeneration [1]. In pancreatic cancer, the TGFB1/INHBA homodimer/Nodal-SMAD2/3 signaling network is a pivotal central node regulating chemoresistance mechanisms, including enhancing stem-like properties of cancer cells and mediating interactions with the stroma [2]. In hematological malignancies, Tgfb1 shows a hematologic-tissue-specific expression pattern, is broadly dysregulated, associated with adverse prognosis, and can predict immunotherapy responses [3].
In summary, Tgfb1 is essential in regulating various biological processes. Mouse models, through genetic disruption of TGF-β signaling, have revealed its significance in intestinal regeneration. In diseases, its dysregulation plays a role in pancreatic cancer chemoresistance and hematological malignancies, highlighting its potential as a therapeutic target in these disease areas.
References:
1. Chen, Lei, Qiu, Xia, Dupre, Abigail, Spence, Jason R, Verzi, Michael P. 2023. TGFB1 induces fetal reprogramming and enhances intestinal regeneration. In Cell stem cell, 30, 1520-1537.e8. doi:10.1016/j.stem.2023.09.015. https://pubmed.ncbi.nlm.nih.gov/37865088/
2. Abdel Mouti, Mai, Pauklin, Siim. 2021. TGFB1/INHBA Homodimer/Nodal-SMAD2/3 Signaling Network: A Pivotal Molecular Target in PDAC Treatment. In Molecular therapy : the journal of the American Society of Gene Therapy, 29, 920-936. doi:10.1016/j.ymthe.2021.01.002. https://pubmed.ncbi.nlm.nih.gov/33429081/
3. Wang, Cui-Zhu, Zhang, Zi-Qi, Zhang, Yan, Sha, Suo, Xu, Zi-Jun. 2023. Comprehensive characterization of TGFB1 across hematological malignancies. In Scientific reports, 13, 19107. doi:10.1038/s41598-023-46552-8. https://pubmed.ncbi.nlm.nih.gov/37925591/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen