C57BL/6NCya-Tuba1aem1/Cya
Common Name
Tuba1a-KO
Product ID
S-KO-05592
Backgroud
C57BL/6NCya
Strain ID
KOCMP-22142-Tuba1a-B6N-VA
When using this mouse strain in a publication, please cite “Tuba1a-KO Mouse (Catalog S-KO-05592) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Tuba1a-KO
Strain ID
KOCMP-22142-Tuba1a-B6N-VA
Gene Name
Product ID
S-KO-05592
Gene Alias
Tuba1, Tuba-1
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 15
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000097014
NCBI RefSeq
NM_011653
Target Region
Exon 1~4
Size of Effective Region
~3.4 kb
Overview of Gene Research
TUBA1A, encoding tubulin alpha 1a, is a crucial gene involved in microtubule assembly. Microtubules, made of α/β-tubulin dimers, are essential for neurite outgrowth, neuronal growth cone responses, and intracellular transport during neurodevelopment [2]. TUBA1A constitutes a major part of α-tubulin in the developing brain [2].
In Tuba1aND/+ mice (a loss-of-function model), α-tubulin levels are reduced in juvenile brains. In postnatal day 0 cultured neurons from these mice, reduced TUBA1A leads to less microtubule assembly in axons, causing more pausing during organelle trafficking. These mice develop adult-onset ataxia, indicating that TUBA1A-rich microtubule tracks are essential for mature neuron function and synapse maintenance [1]. In another study, the Tuba1aND/+ mice showed normal brain formation except for significant impacts on axon extension and impaired formation of forebrain commissures. Neurons with reduced Tuba1a due to the Tuba1aND mutation had slower outgrowth and failed to localize microtubule-associated protein-1b to the growth cone, likely affecting microtubule stabilization [2].
In conclusion, TUBA1A is vital for microtubule-related functions in neuronal development and maintenance. The Tuba1a loss-of-function mouse models have revealed its role in processes such as axon extension, commissure formation, organelle trafficking, and the maintenance of mature neurons and synapses. Mutations in TUBA1A are associated with severe brain malformations and neurological defects, highlighting its importance in understanding related disease mechanisms [1,2].
References:
1. Buscaglia, Georgia, Northington, Kyle R, Moore, Jeffrey K, Bates, Emily Anne. 2020. Reduced TUBA1A Tubulin Causes Defects in Trafficking and Impaired Adult Motor Behavior. In eNeuro, 7, . doi:10.1523/ENEURO.0045-20.2020. https://pubmed.ncbi.nlm.nih.gov/32184299/
2. Buscaglia, Georgia, Northington, Kyle R, Aiken, Jayne, Hoff, Katelyn J, Bates, Emily A. 2022. Bridging the Gap: The Importance of TUBA1A α-Tubulin in Forming Midline Commissures. In Frontiers in cell and developmental biology, 9, 789438. doi:10.3389/fcell.2021.789438. https://pubmed.ncbi.nlm.nih.gov/35127710/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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