C57BL/6JCya-Btbd9em1/Cya
Common Name:
Btbd9-KO
Product ID:
S-KO-05827
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Btbd9-KO
Strain ID
KOCMP-224671-Btbd9-B6J-VA
Gene Name
Product ID
S-KO-05827
Gene Alias
1700023F20Rik; 4930402L05
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Btbd9em1/Cya mice (Catalog S-KO-05827) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000168787
NCBI RefSeq
NM_027060
Target Region
Exon 2~4
Size of Effective Region
~5.6 kb
Detailed Document
Overview of Gene Research
BTBD9, without common aliases noted in the references, is associated with the regulation of the insulin/insulin-like growth factor signaling pathway and has significant importance in neurological function [1]. It is also linked to the ubiquitination and degradation of TNFAIP1, thereby regulating cancer cell migration [6]. Genetic models, especially KO mouse models, have been crucial in understanding its functions.
In knockout models, loss of the BTBD9 homolog in mice (Btbd9) and flies results in RLS-like phenotypes. In Caenorhabditis elegans, loss of hpo-9 (BTBD9 homolog) leads to increased susceptibility to Mn-induced oxidative stress, mitochondrial dysfunction, decreased dopamine levels, and altered dopaminergic neuronal morphology and behavior [1]. In mice, Btbd9 knockout causes enhanced neural activity in the striatum, increased postsynaptic currents in medium spiny neurons, and decreased excitability of cholinergic interneurons. Specific knockout in striatal medium spiny neurons leads to rest-phase motor restlessness, sleep disturbance, and increased thermal sensation [2]. Knockout in the cerebellum affects Purkinje cell activity, causing motor restlessness during the rest phase [3,5]. In the cerebral cortex, knockout results in increased neural activity in certain areas, decreased thickness in parts of the sensorimotor cortex, and enhanced short-term plasticity at corticostriatal terminals, also leading to rest-phase motor restlessness [4].
In conclusion, BTBD9 plays essential roles in regulating oxidative stress, neurotoxicity, and the activity of neurons in multiple brain regions. Btbd9 KO/CKO mouse models have significantly contributed to understanding its role in Restless Legs Syndrome, highlighting its potential as a therapeutic target for this neurological disorder [1-7].
References:
1. Chen, Pan, Cheng, Hong, Zheng, Fuli, Bowman, Aaron B, Aschner, Michael. . BTBD9 attenuates manganese-induced oxidative stress and neurotoxicity by regulating insulin growth factor signaling pathway. In Human molecular genetics, 31, 2207-2222. doi:10.1093/hmg/ddac025. https://pubmed.ncbi.nlm.nih.gov/35134179/
2. Lyu, Shangru, Xing, Hong, DeAndrade, Mark P, Walters, Arthur S, Li, Yuqing. 2019. The Role of BTBD9 in Striatum and Restless Legs Syndrome. In eNeuro, 6, . doi:10.1523/ENEURO.0277-19.2019. https://pubmed.ncbi.nlm.nih.gov/31444227/
3. Lyu, Shangru, Xing, Hong, DeAndrade, Mark P, Walters, Arthur S, Li, Yuqing. 2020. The Role of BTBD9 in the Cerebellum, Sleep-like Behaviors and the Restless Legs Syndrome. In Neuroscience, 440, 85-96. doi:10.1016/j.neuroscience.2020.05.021. https://pubmed.ncbi.nlm.nih.gov/32446853/
4. Lyu, Shangru, Xing, Hong, DeAndrade, Mark P, Febo, Marcelo, Li, Yuqing. 2019. The role of BTBD9 in the cerebral cortex and the pathogenesis of restless legs syndrome. In Experimental neurology, 323, 113111. doi:10.1016/j.expneurol.2019.113111. https://pubmed.ncbi.nlm.nih.gov/31715135/
5. Lyu, Shangru, Xing, Hong, Liu, Yuning, Yokoi, Fumiaki, Li, Yuqing. 2022. Further Studies on the Role of BTBD9 in the Cerebellum, Sleep-like Behaviors and the Restless Legs Syndrome. In Neuroscience, 505, 78-90. doi:10.1016/j.neuroscience.2022.10.008. https://pubmed.ncbi.nlm.nih.gov/36244636/
6. Li, Lihui, Zhang, Wenjuan, Liu, Yue, Wei, Wenyi, Jia, Lijun. 2020. The CRL3BTBD9 E3 ubiquitin ligase complex targets TNFAIP1 for degradation to suppress cancer cell migration. In Signal transduction and targeted therapy, 5, 42. doi:10.1038/s41392-020-0140-z. https://pubmed.ncbi.nlm.nih.gov/32327643/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen