Dus3l, a dihydrouridine synthase (DUS) homolog, is involved in epitranscriptomic RNA regulation through mediating dihydrouridylation [1,2]. Epitranscriptomic RNA modifications regulate RNA activity, and DUS3L's role in this process is crucial for fundamental gene expression processes related to various RNA species [2].

In functional studies, DUS3L-knockout (KO) cells show compromised protein translation rates and impaired cellular proliferation, revealing its significant role in these biological processes [1]. Although no KO/CKO mouse models are mentioned in the references, the cell-based KO experiments provide insights into DUS3L's function.

In conclusion, DUS3L is essential for RNA-mediated dihydrouridylation, which impacts protein translation and cellular proliferation. The KO cell-based research on DUS3L contributes to understanding its role in normal biological functions, and potentially, its implications in diseases where these processes are dysregulated, as DUS3L has been associated with familial inflammatory bowel disease, aortic aneurysm and dissection, and acute myocardial infarction [3,4,5].

References:

1. Dai, Wei, Li, Ang, Yu, Nathan J, Wühr, Martin, Kleiner, Ralph E. 2021. Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation. In Nature chemical biology, 17, 1178-1187. doi:10.1038/s41589-021-00874-8. https://pubmed.ncbi.nlm.nih.gov/34556860/

2. Dai, Wei, Yu, Nathan J, Kleiner, Ralph E. 2023. Chemoproteomic Approaches to Studying RNA Modification-Associated Proteins. In Accounts of chemical research, 56, 2726-2739. doi:10.1021/acs.accounts.3c00450. https://pubmed.ncbi.nlm.nih.gov/37733063/

3. Park, Yoo Min, Ha, Eunji, Gu, Ki-Nam, Kim, Kwangwoo, Kim, Hyo Jong. . Host Genetic and Gut Microbial Signatures in Familial Inflammatory Bowel Disease. In Clinical and translational gastroenterology, 11, e00213. doi:10.14309/ctg.0000000000000213. https://pubmed.ncbi.nlm.nih.gov/32764209/

4. Zhang, Yiran, Li, Lin, Ma, Liang. 2021. Integrative analysis of transcriptome-wide association study and mRNA expression profile identified candidate genes and pathways associated with aortic aneurysm and dissection. In Gene, 808, 145993. doi:10.1016/j.gene.2021.145993. https://pubmed.ncbi.nlm.nih.gov/34626721/

5. Zhang, Baojian, Li, Biao, Sun, Chao, Xiao, Yichao, Liu, Qiming. 2021. Identification of key gene modules and pathways of human platelet transcriptome in acute myocardial infarction patients through co-expression network. In American journal of translational research, 13, 3890-3905. doi:. https://pubmed.ncbi.nlm.nih.gov/34017580/