C57BL/6JCya-Gjd4em1/Cya
Common Name
Gjd4-KO
Product ID
S-KO-05863
Backgroud
C57BL/6JCya
Strain ID
KOCMP-225152-Gjd4-B6J-VA
When using this mouse strain in a publication, please cite “Gjd4-KO Mouse (Catalog S-KO-05863) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Gjd4-KO
Strain ID
KOCMP-225152-Gjd4-B6J-VA
Gene Name
Product ID
S-KO-05863
Gene Alias
Cx39, Cxnu, 9430022F06Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 18
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000041007
NCBI RefSeq
NM_153086
Target Region
Exon 1~2
Size of Effective Region
~2.6 kb
Overview of Gene Research
Gjd4, encoding the Cx46.8 protein, is a connexin gene conserved across vertebrate lineages. Gap junction (GJ) channels formed by connexins mediate bioelectric signaling, and Gjd4-related GJ channels are crucial for slow muscle development and function. They transmit spinal-cord-generated neural activity to developing slow muscle cells and synchronize the activity spread, which is essential for appropriate myofiber organization in the developing neuromuscular system [1,2].
In a study on Cx39-deficient mice (where the coding region of the Gjd4 gene was replaced by eGFP), myogenesis in embryos was accelerated, as indicated by increased myogenin expression and increased Cx43 expression in developing skeletal muscle. Also, the regeneration process of skeletal muscle in these mice was accelerated, with earlier onset of MyoD and myogenin expression. It was hypothesized that Cx43 may compensate for the loss of Cx39 during myogenesis and regeneration [3].
In conclusion, Gjd4 is essential for slow muscle development, function, and myofiber organization through mediating bioelectric signaling. The gene knockout model in mice has revealed its role in myogenesis and skeletal muscle regeneration, contributing to our understanding of developmental myopathies and muscle-related biological processes.
References:
1. Lukowicz-Bedford, Rachel M, Eisen, Judith S, Miller, Adam C. 2024. Gap-junction-mediated bioelectric signaling required for slow muscle development and function in zebrafish. In Current biology : CB, 34, 3116-3132.e5. doi:10.1016/j.cub.2024.06.007. https://pubmed.ncbi.nlm.nih.gov/38936363/
2. Lukowicz-Bedford, R M, Eisen, J S, Miller, A C. 2023. Gap junction mediated bioelectric coordination is required for slow muscle development, organization, and function. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.12.20.572619. https://pubmed.ncbi.nlm.nih.gov/38187655/
3. von Maltzahn, Julia, Wulf, Volker, Matern, Gabi, Willecke, Klaus. 2011. Connexin39 deficient mice display accelerated myogenesis and regeneration of skeletal muscle. In Experimental cell research, 317, 1169-78. doi:10.1016/j.yexcr.2011.01.017. https://pubmed.ncbi.nlm.nih.gov/21272575/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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