C57BL/6JCya-Flvcr1em1/Cya
Common Name
Flvcr1-KO
Product ID
S-KO-06025
Backgroud
C57BL/6JCya
Strain ID
KOCMP-226844-Flvcr1-B6J-VA
When using this mouse strain in a publication, please cite “Flvcr1-KO Mouse (Catalog S-KO-06025) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Flvcr1-KO
Strain ID
KOCMP-226844-Flvcr1-B6J-VA
Gene Name
Product ID
S-KO-06025
Gene Alias
9630055N22Rik, FLVCR, Mfsd7b
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 1
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000085635
NCBI RefSeq
NM_001081259
Target Region
Exon 2
Size of Effective Region
~1.7 kb
Overview of Gene Research
**FLVCR1**, or feline leukemia virus subgroup C cellular receptor 1, is a significant gene. It functions as a plasma-membrane choline transporter in mammals, facilitating the uptake of choline into cells, which is crucial for choline metabolism and the initiation of the Kennedy pathway for phospholipid synthesis [1,2]. FLVCR1 is also associated with maintaining mitochondrial homeostasis and is involved in multiple cellular activities such as proliferation, migration, and invasion in various cancer-related processes [1,2].
Genetic studies, especially knockout (KO) models, have been instrumental in understanding FLVCR1's role. Flvcr1 knockout mice are embryonic lethal, but this can be partially rescued by choline supplementation, indicating its essential role in choline-related biological processes [1]. In cancer research, loss-of-function experiments in cell lines have shown that FLVCR1 knockdown inhibits cell proliferation, migration, and invasion in esophageal squamous cell carcinoma, colorectal cancer, cervical cancer, osteosarcoma, and cholangiocarcinoma [1].
In summary, FLVCR1 is essential for choline metabolism and mitochondrial homeostasis. KO mouse models have demonstrated its critical role in embryonic development related to choline availability. In the context of cancer, loss-of-function studies have revealed its role in promoting the malignant phenotype of various cancer cells, providing potential therapeutic targets for cancer treatment.
References:
1. Kenny, Timothy C, Khan, Artem, Son, Yeeun, Hite, Richard K, Birsoy, Kıvanç. 2023. Integrative genetic analysis identifies FLVCR1 as a plasma-membrane choline transporter in mammals. In Cell metabolism, 35, 1057-1071.e12. doi:10.1016/j.cmet.2023.04.003. https://pubmed.ncbi.nlm.nih.gov/37100056/
2. Son, Yeeun, Kenny, Timothy C, Khan, Artem, Birsoy, Kıvanç, Hite, Richard K. 2024. Structural basis of lipid head group entry to the Kennedy pathway by FLVCR1. In Nature, 629, 710-716. doi:10.1038/s41586-024-07374-4. https://pubmed.ncbi.nlm.nih.gov/38693265/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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