C57BL/6NCya-Nfrkbem1/Cya
Common Name:
Nfrkb-KO
Product ID:
S-KO-06783
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Nfrkb-KO
Strain ID
KOCMP-235134-Nfrkb-B6N-VA
Gene Name
Product ID
S-KO-06783
Gene Alias
A530090G11Rik
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Nfrkbem1/Cya mice (Catalog S-KO-06783) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000086167
NCBI RefSeq
NM_172766
Target Region
Exon 3~12
Size of Effective Region
~9.0 kb
Detailed Document
Overview of Gene Research
Nfrkb, short for nuclear factor related to kappaB binding protein, is a metazoan-specific subunit of the INO80 chromatin remodeling complex. The INO80 complex is crucial for gene transcription regulation, and is involved in DNA repair, replication, and other important cellular biological processes [1].
In human ALT cells, Nfrkb can associate with telomeres. Loss of Nfrkb in U2OS cells leads to telomere dysfunction and fewer PML bodies. Also, it is highly expressed in hepatocellular carcinoma (HCC) cells and its high expression is related to short overall survival time and poor prognosis, suggesting it may be a prognostic biomarker for HCC treatment [2]. In gastric cancer cells, lncRNA DRAIC can interfere with the deubiquitination of Nfrkb mediated by UCHL5, promoting Nfrkb degradation via ubiquitination and thus inhibiting the proliferation and metastasis of gastric cancer cells [3]. In minimal change nephrotic syndrome, Nfrkb is upregulated in relapse, mainly in CD4+T cells and B cells, and promotes hypomethylation of genomic DNA, potentially involved in transcriptional regulation disorders during relapse [4].
In summary, Nfrkb, as a subunit of the INO80 complex, plays important roles in multiple biological processes. Its functions in telomere protection, and its implications in liver and gastric cancers, as well as in minimal change nephrotic syndrome, have been revealed through relevant studies. Understanding Nfrkb can provide insights into these disease mechanisms and potential treatment strategies.
References:
1. Peng, Qiyao, Wan, Dan, Zhou, Rongrong, Huang, Xuekuan, Peng, Yongbo. 2022. The biological function of metazoan-specific subunit nuclear factor related to kappaB binding protein of INO80 complex. In International journal of biological macromolecules, 203, 176-183. doi:10.1016/j.ijbiomac.2022.01.155. https://pubmed.ncbi.nlm.nih.gov/35093437/
2. Peng, Qiyao, Zhou, Mingqing, Zuo, Shanru, He, Zuping, He, Quanyuan. 2021. Nuclear Factor Related to KappaB Binding Protein (NFRKB) Is a Telomere-Associated Protein and Involved in Liver Cancer Development. In DNA and cell biology, 40, 1298-1307. doi:10.1089/dna.2021.0486. https://pubmed.ncbi.nlm.nih.gov/34591601/
3. Zhang, Zheng, Hu, Xiaoxuan, Kuang, Jia, Liao, Jinmao, Yuan, Qi. 2020. LncRNA DRAIC inhibits proliferation and metastasis of gastric cancer cells through interfering with NFRKB deubiquitination mediated by UCHL5. In Cellular & molecular biology letters, 25, 29. doi:10.1186/s11658-020-00221-0. https://pubmed.ncbi.nlm.nih.gov/32351584/
4. Audard, Vincent, Pawlak, André, Candelier, Marina, Lang, Philippe, Sahali, Djillali. 2012. Upregulation of nuclear factor-related kappa B suggests a disorder of transcriptional regulation in minimal change nephrotic syndrome. In PloS one, 7, e30523. doi:10.1371/journal.pone.0030523. https://pubmed.ncbi.nlm.nih.gov/22291976/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen