C57BL/6JCya-Ccdc38em1/Cya
Common Name
Ccdc38-KO
Product ID
S-KO-06898
Backgroud
C57BL/6JCya
Strain ID
KOCMP-237465-Ccdc38-B6J-VA
When using this mouse strain in a publication, please cite “Ccdc38-KO Mouse (Catalog S-KO-06898) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Ccdc38-KO
Strain ID
KOCMP-237465-Ccdc38-B6J-VA
Gene Name
Product ID
S-KO-06898
Gene Alias
4933417K05Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 10
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000092215
NCBI RefSeq
NM_175488
Target Region
Exon 5~11
Size of Effective Region
~15.4 kb
Overview of Gene Research
Ccdc38, the coiled-coil domain-containing 38 gene, is a testes-specific gene in mice, and its protein product is conserved in various mammalian species [2]. It is mainly expressed in spermatogonia and spermatocytes. Functionally, it is involved in sperm flagellum biogenesis, which is essential for male fertility [1].
In male mice with Ccdc38 inactivation, the manchette is distorted, spermatozoa flagella show multiple morphological abnormalities, and eventually, male sterility occurs [1]. Ccdc38 interacts with CCDC42, localizing on the manchette and sperm tail during spermiogenesis [1]. It also interacts with intraflagellar transport protein 88 (IFT88) and outer dense fibrous 2 (ODF2), and Ccdc38 knockout reduces the transport of ODF2 to the flagellum [1]. Additionally, Ccdc38-KO sperm display acrosomal hypoplasia and typical multiple morphological abnormalities of the flagella (MMAF) phenotypes, with disorganized fibrous sheaths [3].
In conclusion, Ccdc38 is crucial for sperm flagellum biogenesis and acrosome biogenesis in mice, as demonstrated by gene-knockout mouse models. These findings suggest that Ccdc38 mutations might be associated with male infertility in humans, providing valuable insights into understanding the molecular mechanisms of male infertility [1,3].
References:
1. Zhang, Ruidan, Wu, Bingbing, Liu, Chao, Yuan, Li, Li, Wei. 2022. CCDC38 is required for sperm flagellum biogenesis and male fertility in mice. In Development (Cambridge, England), 149, . doi:10.1242/dev.200516. https://pubmed.ncbi.nlm.nih.gov/35587122/
2. Lin, Shou-Ren, Li, Yu-Chi, Luo, Man-Ling, Jiang, Zhi-Mao, Gui, Yao-Ting. 2016. Identification and characteristics of the testes-specific gene, Ccdc38, in mice. In Molecular medicine reports, 14, 1290-6. doi:10.3892/mmr.2016.5360. https://pubmed.ncbi.nlm.nih.gov/27278724/
3. Wang, Yaling, Huang, Xueying, Sun, Guoying, Li, Jinsong, Wang, Lingbo. 2023. Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice. In Journal of genetics and genomics = Yi chuan xue bao, 51, 407-418. doi:10.1016/j.jgg.2023.09.002. https://pubmed.ncbi.nlm.nih.gov/37709195/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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