C57BL/6JCya-Mief2em1/Cya
Common Name:
Mief2-KO
Product ID:
S-KO-06914
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mief2-KO
Strain ID
KOCMP-237781-Mief2-B6J-VA
Gene Name
Product ID
S-KO-06914
Gene Alias
Gm11; Smcr7
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mief2em1/Cya mice (Catalog S-KO-06914) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000018743
NCBI RefSeq
NM_001009927
Target Region
Exon 2~4
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
MIEF2, also known as mitochondrial elongation factor 2 or MiD49, is a mitochondrial outer membrane protein that functions as a key regulator of mitochondrial fission [1,3,4,6,7,8]. It is involved in various cellular processes, including metabolism, redox homeostasis, and apoptosis, and has been associated with multiple diseases, such as ovarian cancer, breast cancer, colorectal cancer, and doxorubicin-induced cardiotoxicity [1,2,3,5,6].
In ovarian cancer, MIEF2 promotes lipid synthesis by up-regulating SREBP1 and SREBP2 and their target genes, driving cancer progression through the ROS/AKT/mTOR signaling pathway [1]. In breast cancer, a soft extracellular matrix increases MIEF1/2-dependent mitochondrial fission, leading to redox homeostasis and chemotherapy resistance [2]. In colorectal cancer, low MIEF2 expression enhances oxaliplatin resistance by inhibiting the mitochondrial apoptosis pathway [5]. In cardiomyocytes, Foxo3a targets and suppresses MIEF2, inhibiting mitochondrial fission and protecting against doxorubicin-induced cardiotoxicity [6].
In conclusion, MIEF2 plays a crucial role in mitochondrial fission and is involved in multiple biological processes related to various diseases. Loss-of-function studies in different cell types and in vivo models have revealed its role in cancer progression and chemotherapy resistance, as well as in cardiotoxicity, providing potential therapeutic targets for these diseases.
References:
1. Zhao, Shuhua, Cheng, Lu, Shi, Yuan, Yun, Qinghui, Yang, Hong. 2021. MIEF2 reprograms lipid metabolism to drive progression of ovarian cancer through ROS/AKT/mTOR signaling pathway. In Cell death & disease, 12, 18. doi:10.1038/s41419-020-03336-6. https://pubmed.ncbi.nlm.nih.gov/33414447/
2. Romani, Patrizia, Nirchio, Nunzia, Arboit, Mattia, Martello, Graziano, Dupont, Sirio. 2022. Mitochondrial fission links ECM mechanotransduction to metabolic redox homeostasis and metastatic chemotherapy resistance. In Nature cell biology, 24, 168-180. doi:10.1038/s41556-022-00843-w. https://pubmed.ncbi.nlm.nih.gov/35165418/
3. Zhao, Shuhua, Zhang, Xiaohong, Shi, Yuan, Li, Jia, Yang, Hong. 2020. MIEF2 over-expression promotes tumor growth and metastasis through reprogramming of glucose metabolism in ovarian cancer. In Journal of experimental & clinical cancer research : CR, 39, 286. doi:10.1186/s13046-020-01802-9. https://pubmed.ncbi.nlm.nih.gov/33317572/
4. Xian, Hongxu, Liou, Yih-Cherng. 2019. Loss of MIEF1/MiD51 confers susceptibility to BAX-mediated cell death and PINK1-PRKN-dependent mitophagy. In Autophagy, 15, 2107-2125. doi:10.1080/15548627.2019.1596494. https://pubmed.ncbi.nlm.nih.gov/30894073/
5. Xie, Chaozheng, Li, Kang, Li, Ya, Wang, Wang, Wei, Zhengqiang. 2022. CRISPR-based knockout screening identifies the loss of MIEF2 to enhance oxaliplatin resistance in colorectal cancer through inhibiting the mitochondrial apoptosis pathway. In Frontiers in oncology, 12, 881487. doi:10.3389/fonc.2022.881487. https://pubmed.ncbi.nlm.nih.gov/36106106/
6. Zhou, Luyu, Li, Ruibei, Liu, Cuiyun, Zhao, Yanfang, Wang, Kun. 2017. Foxo3a inhibits mitochondrial fission and protects against doxorubicin-induced cardiotoxicity by suppressing MIEF2. In Free radical biology & medicine, 104, 360-370. doi:10.1016/j.freeradbiomed.2017.01.037. https://pubmed.ncbi.nlm.nih.gov/28137654/
7. Cheng, Zhiyong. . FoxO transcription factors in mitochondrial homeostasis. In The Biochemical journal, 479, 525-536. doi:10.1042/BCJ20210777. https://pubmed.ncbi.nlm.nih.gov/35195252/
8. Liu, Tong, Yu, Rong, Jin, Shao-Bo, Zhao, Jian, Nistér, Monica. 2013. The mitochondrial elongation factors MIEF1 and MIEF2 exert partially distinct functions in mitochondrial dynamics. In Experimental cell research, 319, 2893-904. doi:10.1016/j.yexcr.2013.07.010. https://pubmed.ncbi.nlm.nih.gov/23880462/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen