Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Nlrp5em1/Cya
Common Name:
Nlrp5-KO
Product ID:
S-KO-07109
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Nlrp5-KO
Strain ID
KOCMP-23968-Nlrp5-B6J-VA
Gene Name
Nlrp5
Product ID
S-KO-07109
Gene Alias
Mater; Nalp5; Op1; PAN11
Background
C57BL/6JCya
NCBI ID
23968
Modification
Conventional knockout
Chromosome
7
Phenotype
MGI:1345193
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nlrp5em1/Cya mice (Catalog S-KO-07109) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000015866
NCBI RefSeq
NM_011860
Target Region
Exon 6~11
Size of Effective Region
~18.2 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Nlrp5, a member of the NLR family pyrin domain-containing genes, is a key maternal-effect gene. It is crucial for normal pre-implantation and embryonic development, being part of the subcortical maternal complex (SCMC) [1,2,3,6,8]. It may be involved in processes related to oocyte maturation, fertilization, and early embryonic development, with potential links to infertility-related pathways [2,4,5,6,8]. Genetic models, such as knockout mouse models, have been valuable in studying its function.

In sows, knockdown of NLRP5 using RNA interference arrested early embryonic development, suggesting its essential role in zygotic genome activation [7]. In mice, Nlrp5 mutant oocytes showed abnormal mitochondrial localization, increased mitochondrial activity, reactive oxygen species accumulation, and ultimately mitochondrial depletion, leading to embryo arrest at the two-cell stage [9]. In humans, mutations in NLRP5 have been identified in patients with early embryonic arrest, oocyte maturation abnormality, and female infertility [2,5,8]. For instance, six novel NLRP5 variants were found in patients with arrested and severely fragmented embryos [2], and a novel homozygous frameshift variant in NLRP5 was associated with oocyte maturation abnormality [5].

In conclusion, Nlrp5 is essential for early embryogenesis, with its function manifested through mitochondrial regulation in oocytes and embryos. Research using gene-knockout models, both in mice and other species, has revealed its critical role in early embryonic development, and its mutations are associated with human infertility and early embryonic arrest. This understanding contributes to the study of reproductive diseases and may offer potential targets for diagnosis and treatment.

References:

1. Huang, Xingchen, Sun, Qinqiang, Chen, Dongrong, Zhang, Ming, Fu, Qiang. 2022. Nlrp5 and Tle6 expression patterns in buffalo oocytes and pre-implantation embryos. In Reproduction in domestic animals = Zuchthygiene, 57, 481-488. doi:10.1111/rda.14084. https://pubmed.ncbi.nlm.nih.gov/35044003/

2. Tong, Xiaomei, Jin, Jiamin, Hu, Zhanhong, Zhang, Yin-Li, Zhang, Songying. 2022. Mutations in OOEP and NLRP5 identified in infertile patients with early embryonic arrest. In Human mutation, 43, 1909-1920. doi:10.1002/humu.24448. https://pubmed.ncbi.nlm.nih.gov/35946397/

3. Unoki, Motoko, Uemura, Shuhei, Fujimoto, Akihiro, Sasaki, Hiroyuki. . The maternal protein NLRP5 stabilizes UHRF1 in the cytoplasm: implication for the pathogenesis of multilocus imprinting disturbance. In Human molecular genetics, 33, 1575-1583. doi:10.1093/hmg/ddae096. https://pubmed.ncbi.nlm.nih.gov/38868925/

4. Xue, Yamei, Cheng, Xiaohong, Xiong, Yuping, Li, Kun. 2022. Gene mutations associated with fertilization failure after in vitro fertilization/intracytoplasmic sperm injection. In Frontiers in endocrinology, 13, 1086883. doi:10.3389/fendo.2022.1086883. https://pubmed.ncbi.nlm.nih.gov/36589837/

5. Huang, Lingli, Wang, Yu, Lu, Fangting, Jin, Rentao, Tong, Xianhong. 2022. Novel mutations in NLRP5 and PATL2 cause female infertility characterized by primarily oocyte maturation abnormality and consequent early embryonic arrest. In Journal of assisted reproduction and genetics, 39, 711-718. doi:10.1007/s10815-022-02412-4. https://pubmed.ncbi.nlm.nih.gov/35091966/

6. Sang, Qing, Zhou, Zhou, Mu, Jian, Wang, Lei. 2021. Genetic factors as potential molecular markers of human oocyte and embryo quality. In Journal of assisted reproduction and genetics, 38, 993-1002. doi:10.1007/s10815-021-02196-z. https://pubmed.ncbi.nlm.nih.gov/33895934/

7. Peng, Hui, Liu, Fang, Li, Wenhao, Zhang, Wenchang. 2015. Knockdown of NLRP5 arrests early embryogenesis in sows. In Animal reproduction science, 163, 151-6. doi:10.1016/j.anireprosci.2015.11.004. https://pubmed.ncbi.nlm.nih.gov/26585895/

8. Xu, Yao, Qian, Ying, Liu, Yu, Xue, Songguo, Sun, Lihua. 2020. A novel homozygous variant in NLRP5 is associate with human early embryonic arrest in a consanguineous Chinese family. In Clinical genetics, 98, 69-73. doi:10.1111/cge.13744. https://pubmed.ncbi.nlm.nih.gov/32222962/

9. Fernandes, Roxanne, Tsuda, Chiharu, Perumalsamy, Alagammal L, Nelson, Lawrence M, Jurisicova, Andrea. 2012. NLRP5 mediates mitochondrial function in mouse oocytes and embryos. In Biology of reproduction, 86, 138, 1-10. doi:10.1095/biolreprod.111.093583. https://pubmed.ncbi.nlm.nih.gov/22357545/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest