C57BL/6JCya-Napepldem1/Cya
Common Name
Napepld-KO
Product ID
S-KO-07410
Backgroud
C57BL/6JCya
Strain ID
KOCMP-242864-Napepld-B6J-VA
When using this mouse strain in a publication, please cite “Napepld-KO Mouse (Catalog S-KO-07410) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Napepld-KO
Strain ID
KOCMP-242864-Napepld-B6J-VA
Gene Name
Product ID
S-KO-07410
Gene Alias
Mbldc1, NAPE-PLD, A530089G06
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 5
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000060899
NCBI RefSeq
NM_178728
Target Region
Exon 3
Size of Effective Region
~1.1 kb
Overview of Gene Research
Napepld, or N-acyl phosphatidylethanolamine phospholipase D, is an enzyme of the endocannabinoid system. It catalyzes the production of N-acylethanolamines (NAEs), a family of endogenous bioactive lipids involved in various biological processes, from neuronal functions to energy homeostasis and feeding behaviors [1,2,3].
In a murine model with an inducible Napepld deletion in intestinal epithelial cells (NapepldΔIEC), the mice displayed lower hypothalamic levels of N-oleoylethanolamine (OEA) in response to high-fat-diet (HFD), along with lower mRNA expression of anorexigenic Pomc in the hypothalamus after lipid challenge, suggesting an impairment of the gut-brain axis in regulating appetite [2]. In another study, region-specific knock-down of Napepld in the ventral tegmental area (VTA) enhanced food-reward seeking and reinforced behaviors, increased in vivo DA release in response to rewards, and protected against high-fat diet-mediated body fat gain and obesity-associated metabolic features [3].
In conclusion, Napepld plays a crucial role in regulating appetite via the gut-brain axis and in controlling reward-related behaviors and energy homeostasis. Mouse models with Napepld deletion or knockdown have revealed its significance in obesity-related metabolic conditions, providing insights into potential therapeutic targets for obesity and associated metabolic disorders [2,3].
References:
1. Minor, K M, Letko, A, Becker, D, Mickelson, J R, Drögemüller, C. 2018. Canine NAPEPLD-associated models of human myelin disorders. In Scientific reports, 8, 5818. doi:10.1038/s41598-018-23938-7. https://pubmed.ncbi.nlm.nih.gov/29643404/
2. Rastelli, Marialetizia, Van Hul, Matthias, Terrasi, Romano, Muccioli, Giulio G, Cani, Patrice D. 2020. Intestinal NAPE-PLD contributes to short-term regulation of food intake via gut-to-brain axis. In American journal of physiology. Endocrinology and metabolism, 319, E647-E657. doi:10.1152/ajpendo.00146.2020. https://pubmed.ncbi.nlm.nih.gov/32776827/
3. Castel, Julien, Li, Guangping, Onimus, Oriane, Luquet, Serge, Gangarossa, Giuseppe. 2024. NAPE-PLD in the ventral tegmental area regulates reward events, feeding and energy homeostasis. In Molecular psychiatry, 29, 1478-1490. doi:10.1038/s41380-024-02427-6. https://pubmed.ncbi.nlm.nih.gov/38361126/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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