C57BL/6NCya-Styk1em1/Cya
Common Name
Styk1-KO
Product ID
S-KO-07457
Backgroud
C57BL/6NCya
Strain ID
KOCMP-243659-Styk1-B6N-VA
When using this mouse strain in a publication, please cite “Styk1-KO Mouse (Catalog S-KO-07457) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Styk1-KO
Strain ID
KOCMP-243659-Styk1-B6N-VA
Gene Name
Product ID
S-KO-07457
Gene Alias
Nok, 9130025L13
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 6
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000049150
NCBI RefSeq
NM_172891
Target Region
Exon 2~4
Size of Effective Region
~2.7 kb
Overview of Gene Research
Styk1, also known as serine/threonine/tyrosine kinase 1 and NOK, is a receptor protein-tyrosine kinase-like molecule. It plays crucial roles in multiple biological processes, with its expression detected in several human organs. Styk1 is involved in pathways related to cell cycle, autophagy, and migration, and is of great importance in tumorigenesis and metastasis in various cancers [1-8]. Genetic models, such as gene-knockout mouse models, have been instrumental in studying its functions.
In gene-knockout studies, Styk1-deleted mice were generated using CRISPR/Cas9 technology. Surprisingly, deletion of Styk1 promoted tumor progression in multiple tumor models, including NK-cell-specific target cell clearance, subcutaneous and intravenous injection of B16F10 melanoma, and the spontaneous breast cancer model MMTV-PyMT. This was accompanied by a reduction of NK cell accumulation in tumor tissues. RNA-seq analysis revealed disparities in migration-and adhesion-related signaling pathways in Styk1-deficient NK cells, with a significant reduction in CCR2 expression. Also, over-expression of Styk1 in cell lines led to mitotic arrest and cytokinesis defects, and depletion of APC3 caused accumulation of Styk1 and G2/M arrest [1,2].
In conclusion, Styk1 plays a key role in mediating NK cell anti-tumor response through regulating CCR2 and trafficking. It also affects cell cycle late mitosis. The study of Styk1-KO mouse models has provided valuable insights into its functions in tumor progression, highlighting its potential as a target for cancer treatment [1,2].
References:
1. He, Junming, He, Yuexi, Biao, Ruojia, Dong, Zhongjun, Du, Juan. 2024. STYK1 mediates NK cell anti-tumor response through regulating CCR2 and trafficking. In Journal of translational medicine, 22, 943. doi:10.1186/s12967-024-05718-2. https://pubmed.ncbi.nlm.nih.gov/39415235/
2. Zeng, Su-Lin, Patel, Suraj S, Lv, Mo-Qi, Shen, Wen H, Liu, Li. 2022. STYK1/NOK affects cell cycle late mitosis and directly interacts with anaphase-promoting complex activator CDH1. In Heliyon, 8, e12058. doi:10.1016/j.heliyon.2022.e12058. https://pubmed.ncbi.nlm.nih.gov/36506394/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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