C57BL/6JCya-Gpr101em1/Cya
Common Name
Gpr101-KO
Product ID
S-KO-07587
Backgroud
C57BL/6JCya
Strain ID
KOCMP-245424-Gpr101-B6J-VA
When using this mouse strain in a publication, please cite “Gpr101-KO Mouse (Catalog S-KO-07587) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Gpr101-KO
Strain ID
KOCMP-245424-Gpr101-B6J-VA
Gene Name
Product ID
S-KO-07587
Gene Alias
GPCR6, Gm365
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr X
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000057645
NCBI RefSeq
NM_001033360
Target Region
Exon 2
Size of Effective Region
~1.9 kb
Overview of Gene Research
GPR101, an orphan G protein-coupled receptor belonging to Family A rhodopsin-like family, is predominantly expressed in the hypothalamus and pituitary. It is actively involved in energy homeostasis and is crucial in regulating functions like energy balance and reproduction [1,2]. GPR101 couples to Gαs, Gαi, Gq/11 and G12/13 proteins and is associated with the regulation of the hypothalamus-pituitary-gonad axis [1].
In transgenic mice with elevated somatotrope Gpr101 expression (GhrhrGpr101), Gpr101 causes elevated GH/prolactin secretion without hyperplasia/tumorigenesis, indicating its role in the regulation of the somatotrope axis function [4]. In macrophages, GPR101 knockdown limited the ability of RvD5n-3 DPA to upregulate cyclic adenosine monophosphate, phagocytosis of bacteria, and efferocytosis, suggesting its role in mediating the leukocyte-directed actions of RvD5n-3 DPA [3].
In conclusion, GPR101 plays essential roles in energy homeostasis, regulation of the somatotrope axis, and mediating leukocyte-directed actions. The study of Gpr101 through mouse models has provided insights into its functions in growth-related and inflammatory processes, which may be relevant to understanding diseases such as X-linked acrogigantism and inflammatory arthritis [1,3,4].
References:
1. Hou, Zhi-Shuai, Tao, Ya-Xiong. 2018. Mutations in GPR101 as a potential cause of X-linked acrogigantism and acromegaly. In Progress in molecular biology and translational science, 161, 47-67. doi:10.1016/bs.pmbts.2018.10.003. https://pubmed.ncbi.nlm.nih.gov/30711029/
2. Yang, Zhao, Wang, Jun-Yan, Yang, Fan, Gao, Ning, Sun, Jin-Peng. 2023. Structure of GPR101-Gs enables identification of ligands with rejuvenating potential. In Nature chemical biology, 20, 484-492. doi:10.1038/s41589-023-01456-6. https://pubmed.ncbi.nlm.nih.gov/37945893/
3. Flak, Magdalena B, Koenis, Duco S, Sobrino, Agua, Palmas, Francesco, Dalli, Jesmond. . GPR101 mediates the pro-resolving actions of RvD5n-3 DPA in arthritis and infections. In The Journal of clinical investigation, 130, 359-373. doi:10.1172/JCI131609. https://pubmed.ncbi.nlm.nih.gov/31793912/
4. Abboud, Dayana, Daly, Adrian F, Dupuis, Nadine, Beckers, Albert, Hanson, Julien. 2020. GPR101 drives growth hormone hypersecretion and gigantism in mice via constitutive activation of Gs and Gq/11. In Nature communications, 11, 4752. doi:10.1038/s41467-020-18500-x. https://pubmed.ncbi.nlm.nih.gov/32958754/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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