C57BL/6NCya-Gm614em1/Cya
Common Name:
Gm614-KO
Product ID:
S-KO-07602
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Gm614-KO
Strain ID
KOCMP-245536-Gm614-B6N-VA
Gene Name
Product ID
S-KO-07602
Gene Alias
--
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
X
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Gm614em1/Cya mice (Catalog S-KO-07602) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000101358
NCBI RefSeq
NM_001033362
Target Region
Exon 1~6
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
Gm614 is a novel uncharacterized protein. It is highly expressed in germinal center (GC) B cells from lupus-prone mice. Its essential function is to protect GC B cells from death, and it does so by suppressing caspase-1 transcription. This regulation is associated with the cell proliferation and death pathways in autoimmune diseases [1].
In a study using gene knockout models, ablation of the GC B-cell-specific Gm614 promoted GC B-cell death and mitigation of autoimmune symptoms. Conversely, overexpression protected GC B cells from death and exacerbated autoimmune symptoms. Mechanistically, nuclear-localized Gm614 reduces caspase-1 expression in GC B cells by binding with the caspase-1 promoter to suppress its activation [1].
In conclusion, Gm614 plays a crucial role in protecting GC B cells from death via suppressing caspase-1 transcription in autoimmune diseases. The gene knockout mouse models have been instrumental in revealing its role in the context of autoimmune symptoms, providing insights into the cell proliferation mechanisms involved in autoimmune diseases.
References:
1. He, Youdi, Xu, Ruonan, Zhai, Bing, Wang, Xiaoqian, Wang, Renxi. 2020. Gm614 Protects Germinal Center B Cells From Death by Suppressing Caspase-1 Transcription in Lupus-Prone Mice. In Frontiers in immunology, 11, 585726. doi:10.3389/fimmu.2020.585726. https://pubmed.ncbi.nlm.nih.gov/33193409/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen