C57BL/6JCya-Pcdh11xem1/Cya
Common Name:
Pcdh11x-KO
Product ID:
S-KO-07605
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Pcdh11x-KO
Strain ID
KOCMP-245578-Pcdh11x-B6J-VA
Gene Name
Product ID
S-KO-07605
Gene Alias
PCDHX; PCDHX11; Pcdh11
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pcdh11xem1/Cya mice (Catalog S-KO-07605) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000113358
NCBI RefSeq
NM_001271810
Target Region
Exon 2
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Pcdh11x, short for protocadherin 11 X-linked protein, is a cell adhesion molecule of the δ1-protocadherin family [4]. It has been implicated in multiple biological processes. In the nervous system, it is involved in circuit formation, and it may also play a role in pathways related to cell migration and invasion. Pcdh11x is of particular interest in understanding brain development and certain diseases [4]. Genetic models, such as KO mouse models, are valuable for studying its functions.
Using Nuclease technology genome editing to disrupt the Pcdh11x gene in mice, it was found that although mossy fiber (MF) sprouting in the adult hippocampus still developed, its target specificity was altered, with new synapses frequently formed on granule cell somata in addition to dendrites. This shows that Pcdh11x controls target specification of MF sprouting [1]. In cultured cortical neurons from embryonic day 16 mice, gain-of-function and loss-of-function experiments revealed that Pcdh11x negatively regulates dendritic branching. Overexpression of wild-type Pcdh11x reduced dendritic complexity, while knockdown of Pcdh11x increased dendritic branching, and it activates PI3K/AKT signaling to achieve this negative regulation [3].
In conclusion, Pcdh11x is crucial for proper nervous system development, regulating processes like dendritic branching and target specification of MF sprouting. The use of gene-knockout mouse models has been instrumental in uncovering these functions, which contribute to our understanding of brain development and related disorders. Additionally, Pcdh11x may have potential implications in cancer immunotherapy, as its mutation might serve as a specific biomarker to predict the efficacy of immune checkpoint inhibitors in lung adenocarcinoma patients [2].
References:
1. Luo, Wenshu, Cruz-Ochoa, Natalia Andrea, Seng, Charlotte, Lukacsovich, Tamás, Földy, Csaba. 2022. Pcdh11x controls target specification of mossy fiber sprouting. In Frontiers in neuroscience, 16, 888362. doi:10.3389/fnins.2022.888362. https://pubmed.ncbi.nlm.nih.gov/36117624/
2. Liu, Manjiao, Yang, Meijia, Zhang, Bei, Guo, Hao, Zhao, Jie. 2024. PCDH11X mutation as a potential biomarker for immune checkpoint therapies in lung adenocarcinoma. In Journal of molecular medicine (Berlin, Germany), 102, 899-912. doi:10.1007/s00109-024-02450-8. https://pubmed.ncbi.nlm.nih.gov/38739269/
3. Wu, Cuiying, Niu, Lijun, Yan, Zhongjie, Zhang, Peng, Xu, Ruxiang. 2015. Pcdh11x Negatively Regulates Dendritic Branching. In Journal of molecular neuroscience : MN, 56, 822-828. doi:10.1007/s12031-015-0515-8. https://pubmed.ncbi.nlm.nih.gov/25687328/
4. Priddle, Thomas H, Crow, Tim J. 2012. Protocadherin 11X/Y a human-specific gene pair: an immunohistochemical survey of fetal and adult brains. In Cerebral cortex (New York, N.Y. : 1991), 23, 1933-41. doi:10.1093/cercor/bhs181. https://pubmed.ncbi.nlm.nih.gov/22744706/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen