C57BL/6JCya-Abcg2em1/Cya
Common Name:
Abcg2-KO
Product ID:
S-KO-08449
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Abcg2-KO
Strain ID
KOCMP-26357-Abcg2-B6J-VA
Gene Name
Product ID
S-KO-08449
Gene Alias
ABC15; ABCP; BCRP; Bcrp1; MXR; MXR1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Abcg2em1/Cya mice (Catalog S-KO-08449) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031822
NCBI RefSeq
NM_011920
Target Region
Exon 3~5
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
ABCG2, also known as breast cancer resistance protein (BCRP), is an ATP-binding cassette transporter. It functions as a poly-specific efflux pump, transporting a wide range of substrates including xenobiotics, drugs, and metabolites out of cells. It is involved in multiple biological processes such as protecting the body from harmful substances by acting as a part of self-defence mechanisms in tissues like the intestine, blood-brain barrier, and placenta [3,5,6]. It also plays a role in drug pharmacokinetics and is linked to multidrug resistance in tumors [2,4,5,6]. Genetic models like knockout mouse models are valuable for studying its functions.
In macrophages, ABCG2 exports itaconate from the cytosol to the extracellular space in an ATPase-dependent manner. ABCG2-mediated itaconate export limits transcription factor TFEB-dependent lysosomal biogenesis and antibacterial innate immunity. In a mouse model of S. typhimurium infection, deficiency of ABCG2-mediated itaconate export in macrophages promotes antibacterial innate immune defense [1].
In conclusion, ABCG2 is a crucial transporter with diverse functions. Model-based research, especially using KO mouse models, has revealed its role in limiting antibacterial innate immunity in macrophages. Understanding ABCG2 functions has potential implications for treating bacterial infections and also for addressing issues like multidrug resistance in acute myeloid leukemia and other cancers [1,2].
References:
1. Chen, Chao, Zhang, Zhenxing, Liu, Caiyun, Liu, Ping, Li, Xinjian. 2024. ABCG2 is an itaconate exporter that limits antibacterial innate immunity by alleviating TFEB-dependent lysosomal biogenesis. In Cell metabolism, 36, 498-510.e11. doi:10.1016/j.cmet.2023.12.015. https://pubmed.ncbi.nlm.nih.gov/38181789/
2. Damiani, Daniela, Tiribelli, Mario. 2023. ABCG2 in Acute Myeloid Leukemia: Old and New Perspectives. In International journal of molecular sciences, 24, . doi:10.3390/ijms24087147. https://pubmed.ncbi.nlm.nih.gov/37108308/
3. Robey, Robert W, To, Kenneth K K, Polgar, Orsolya, Dean, Michael, Bates, Susan E. 2008. ABCG2: a perspective. In Advanced drug delivery reviews, 61, 3-13. doi:10.1016/j.addr.2008.11.003. https://pubmed.ncbi.nlm.nih.gov/19135109/
4. Chandratre, Sharayu, Olsen, Jordyn, Howley, Richard, Chen, Bin. 2023. Targeting ABCG2 transporter to enhance 5-aminolevulinic acid for tumor visualization and photodynamic therapy. In Biochemical pharmacology, 217, 115851. doi:10.1016/j.bcp.2023.115851. https://pubmed.ncbi.nlm.nih.gov/37858868/
5. Wiese, Michael. 2015. BCRP/ABCG2 inhibitors: a patent review (2009-present). In Expert opinion on therapeutic patents, 25, 1229-37. doi:10.1517/13543776.2015.1076796. https://pubmed.ncbi.nlm.nih.gov/26291461/
6. Hasanabady, Maryam Hosseini, Kalalinia, Fatemeh. . ABCG2 inhibition as a therapeutic approach for overcoming multidrug resistance in cancer. In Journal of biosciences, 41, 313-24. doi:. https://pubmed.ncbi.nlm.nih.gov/27240991/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen