C57BL/6JCya-Fgd2em1/Cya
Common Name
Fgd2-KO
Product ID
S-KO-08472
Backgroud
C57BL/6JCya
Strain ID
KOCMP-26382-Fgd2-B6J-VA
When using this mouse strain in a publication, please cite “Fgd2-KO Mouse (Catalog S-KO-08472) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Fgd2-KO
Strain ID
KOCMP-26382-Fgd2-B6J-VA
Gene Name
Product ID
S-KO-08472
Gene Alias
Tcd2, tcs2, Tcd-2, tcs-2, ZFYVE4
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 17
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000024810
NCBI RefSeq
NM_013710
Target Region
Exon 2~8
Size of Effective Region
~4.6 kb
Overview of Gene Research
Fgd2, a member of the FGD family, encodes a Rho guanine nucleotide exchange factor. It contains a Dbl homology (DH) domain responsible for guanine nucleotide exchange of CDC42, activating downstream factors, and two pleckstrin homology domains segregated by a FYVE domain [1,4]. By specifically activating Cdc42, Fgd2 controls cytoskeleton-dependent membrane rearrangements and is involved in leukocyte signaling and vesicle trafficking in antigen-presenting cells [2]. It also plays a role in embryonic development as its transcripts are present in diverse tissues during mouse embryogenesis [4].
A loss-of-function allele of Fgd2 generated by gene targeting reduced the transmission ratio of the t haplotype t(h49), directly demonstrating its role as a Distorter in transmission ratio distortion (TRD) in mice. This shows that Fgd2 identifies a second Rho G protein signaling pathway promoting TRD [3].
In conclusion, Fgd2 is crucial for regulating cytoskeleton-related processes, embryonic development, and sperm-related inheritance mechanisms like TRD. The gene knockout mouse model has been valuable in revealing its role in TRD, providing insights into the genetic mechanisms underlying this reproductive phenomenon.
References:
1. Chuan, Junlan, He, Shiyu, Xie, Tian, Wang, Ganggang, Yang, Zhenglin. 2020. Characterization of guanine nucleotide exchange activity of DH domain of human FGD2. In Protein expression and purification, 176, 105693. doi:10.1016/j.pep.2020.105693. https://pubmed.ncbi.nlm.nih.gov/32681954/
2. Huber, Christoph, Mårtensson, Annica, Bokoch, Gary M, Nemazee, David, Gavin, Amanda L. 2008. FGD2, a CDC42-specific exchange factor expressed by antigen-presenting cells, localizes to early endosomes and active membrane ruffles. In The Journal of biological chemistry, 283, 34002-12. doi:10.1074/jbc.M803957200. https://pubmed.ncbi.nlm.nih.gov/18838382/
3. Bauer, Hermann, Véron, Nathalie, Willert, Jürgen, Herrmann, Bernhard G. . The t-complex-encoded guanine nucleotide exchange factor Fgd2 reveals that two opposing signaling pathways promote transmission ratio distortion in the mouse. In Genes & development, 21, 143-7. doi:. https://pubmed.ncbi.nlm.nih.gov/17234881/
4. Pasteris, N G, Gorski, J L. . Isolation, characterization, and mapping of the mouse and human Fgd2 genes, faciogenital dysplasia (FGD1; Aarskog syndrome) gene homologues. In Genomics, 60, 57-66. doi:. https://pubmed.ncbi.nlm.nih.gov/10458911/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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