C57BL/6JCya-Bahcc1em1/Cya
Common Name:
Bahcc1-KO
Product ID:
S-KO-08577
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Bahcc1-KO
Strain ID
KOCMP-268515-Bahcc1-B6J-VA
Gene Name
Product ID
S-KO-08577
Gene Alias
B930044J06
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bahcc1em1/Cya mice (Catalog S-KO-08577) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000122148
NCBI RefSeq
NM_001347621
Target Region
Exon 3~6
Size of Effective Region
~14.4 kb
Detailed Document
Overview of Gene Research
Bahcc1, or Bromo-adjacent homology domain and coiled-coil containing 1, is a gene with significant roles in multiple biological processes. It encodes a protein that can act as a reader molecule of trimethylated histone H3 lysine 27 (H3K27me3), which is involved in regulating gene repression, cell-fate determination, and differentiation [3,4]. It also participates in various cellular pathways such as cell-cycle regulation, DNA repair, and epigenetic regulation, thereby being of great biological importance. Genetic models, especially mouse models, have been crucial in understanding its functions.
In melanoma, super-enhancer-driven expression of BAHCC1 promotes cell proliferation and genome stability. BAHCC1 silencing leads to decreased cell proliferation and delayed DNA repair, and its deficiency can cooperate with PARP inhibition to induce melanoma cell death [1]. In a mouse model of MLL-ENL-mediated leukemia, Bahcc1 is critical for the aberrant epigenetic program. MLL-ENL upregulates Bahcc1, and depletion of Bahcc1 in mice suppresses the leukemogenic activity of MLL-ENL [2]. Also, disruption of the BAHCC1-H3K27me3 interaction in leukemia causes derepression of H3K27me3-targeted genes involved in tumor suppression and cell differentiation, suppressing oncogenesis. In mice, a germline mutation at Bahcc1 to disrupt its H3K27me3 engagement causes partial postnatal lethality, indicating its role in development [3].
In summary, Bahcc1 plays essential roles in regulating cell-cycle, DNA repair, and epigenetic modification processes. Through gene knockout or conditional knockout mouse models, its significance in diseases such as melanoma and leukemia has been revealed. These findings contribute to understanding the biological functions of Bahcc1 and provide potential therapeutic targets for related diseases.
References:
1. Berico, Pietro, Nogaret, Maguelone, Cigrang, Max, Davidson, Irwin, Coin, Frédéric. 2023. Super-enhancer-driven expression of BAHCC1 promotes melanoma cell proliferation and genome stability. In Cell reports, 42, 113363. doi:10.1016/j.celrep.2023.113363. https://pubmed.ncbi.nlm.nih.gov/37924516/
2. Nakamura, Akihide, Masuya, Masahiro, Shinmei, Makoto, Nosaka, Tetsuya, Ono, Ryoichi. . Bahcc1 is critical for the aberrant epigenetic program in a mouse model of MLL-ENL-mediated leukemia. In Blood advances, 8, 2193-2206. doi:10.1182/bloodadvances.2023011320. https://pubmed.ncbi.nlm.nih.gov/38452334/
3. Fan, Huitao, Lu, Jiuwei, Guo, Yiran, Song, Jikui, Wang, Gang Greg. 2020. BAHCC1 binds H3K27me3 via a conserved BAH module to mediate gene silencing and oncogenesis. In Nature genetics, 52, 1384-1396. doi:10.1038/s41588-020-00729-3. https://pubmed.ncbi.nlm.nih.gov/33139953/
4. Guo, Yiran, Zhao, Shuai, Wang, Gang Greg. 2021. Polycomb Gene Silencing Mechanisms: PRC2 Chromatin Targeting, H3K27me3 'Readout', and Phase Separation-Based Compaction. In Trends in genetics : TIG, 37, 547-565. doi:10.1016/j.tig.2020.12.006. https://pubmed.ncbi.nlm.nih.gov/33494958/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen