C57BL/6JCya-Cnpy1em1/Cya
Common Name
Cnpy1-KO
Product ID
S-KO-08710
Backgroud
C57BL/6JCya
Strain ID
KOCMP-269637-Cnpy1-B6J-VA
When using this mouse strain in a publication, please cite “Cnpy1-KO Mouse (Catalog S-KO-08710) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Cnpy1-KO
Strain ID
KOCMP-269637-Cnpy1-B6J-VA
Gene Name
Product ID
S-KO-08710
Gene Alias
1500012D20Rik, 9630008K15Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 5
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000118882
NCBI RefSeq
NM_001310511
Target Region
Exon 1~3
Size of Effective Region
~6.4 kb
Overview of Gene Research
Cnpy1, also known as Canopy1, is a member of the Canopy family proteins. It is a Saposin-related protein and is involved in regulating FGF signaling pathways. This regulation is of great biological importance as it impacts various developmental processes [1,2].
In zebrafish, knockdown of cnpy1 led to midbrain-hindbrain boundary (MHB) defects and impaired FGF signaling in a cell-autonomous manner. Cnpy1 is localized in the endoplasmic reticulum and interacts with FGFR1, highlighting a positive-feedback loop between the FGFR pathway and Cnpy1 that ensures the strength of FGF signaling in the MHB, crucial for the correct development of the tectum and cerebellum [1]. Additionally, in zebrafish, Cnpy1 positively regulates FGF signals within dorsal forerunner cells (DFCs), promoting Cadherin1-mediated cell adhesion between adjacent DFCs to sustain cell cluster formation. Disrupting this FGF positive-feedback loop results in DFC cluster failure, KV malformation, and defects in establishing laterality [2].
In conclusion, Cnpy1 plays essential roles in development, especially in processes related to FGF signaling-mediated organogenesis such as in the MHB and Kupffer's vesicle formation. The gene knockout-like studies in zebrafish have provided valuable insights into its function, highlighting its significance in normal development and potentially in related disease conditions if its regulation is disrupted.
References:
1. Hirate, Yoshikazu, Okamoto, Hitoshi. . Canopy1, a novel regulator of FGF signaling around the midbrain-hindbrain boundary in zebrafish. In Current biology : CB, 16, 421-7. doi:. https://pubmed.ncbi.nlm.nih.gov/16488878/
2. Matsui, Takaaki, Thitamadee, Siripong, Murata, Tomoko, Okamoto, Hitoshi, Bessho, Yasumasa. 2011. Canopy1, a positive feedback regulator of FGF signaling, controls progenitor cell clustering during Kupffer's vesicle organogenesis. In Proceedings of the National Academy of Sciences of the United States of America, 108, 9881-6. doi:10.1073/pnas.1017248108. https://pubmed.ncbi.nlm.nih.gov/21628557/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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