C57BL/6JCya-Nlrx1em1/Cya
Common Name
Nlrx1-KO
Product ID
S-KO-08765
Backgroud
C57BL/6JCya
Strain ID
KOCMP-270151-Nlrx1-B6J-VA
When using this mouse strain in a publication, please cite “Nlrx1-KO Mouse (Catalog S-KO-08765) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Nlrx1-KO
Strain ID
KOCMP-270151-Nlrx1-B6J-VA
Gene Name
Product ID
S-KO-08765
Gene Alias
NOD9
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 9
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000034621
NCBI RefSeq
NM_178420
Target Region
Exon 4~5
Size of Effective Region
~1.6 kb
Overview of Gene Research
NLRX1, also known as NLR family member X1, is a member of the Nod-like receptor (NLR) family. It localizes to the mitochondrial matrix in resting conditions and has been proposed to regulate multiple cellular processes, including antiviral immunity, apoptosis, ROS generation, mitochondrial metabolism, and mitophagy [2]. It is involved in various signaling pathways such as negatively regulating type-I interferon, attenuating pro-inflammatory NF-κB signaling, and modulating autophagy [3].
In studies related to intervertebral disc degeneration (IDD), the loss of NLRX1 was correlated with nucleus pulposus (NP) cell senescence and IDD progression, involving disordered mitochondrial quality. NLRX1-defective NP cells showed mitochondrial collapse, excessive mitophagy, and aggressive NP cell senescence [1]. In macrophages, NLRX1 knockout (KO) mice had enhanced M2 macrophage polarization and excessive renal fibrosis in chronic obstructive nephropathy, indicating NLRX1's role in preventing such pathological changes [4].
In conclusion, NLRX1 plays essential biological functions in regulating mitochondrial dynamics, mitophagy, immune responses, and cell metabolism. Studies using NLRX1 KO mouse models have revealed its significance in diseases like IDD and chronic obstructive nephropathy, providing insights into disease mechanisms and potential therapeutic targets.
References:
1. Song, Yu, Liang, Huaizhen, Li, Gaocai, Wang, Kun, Yang, Cao. 2023. The NLRX1-SLC39A7 complex orchestrates mitochondrial dynamics and mitophagy to rejuvenate intervertebral disc by modulating mitochondrial Zn2+ trafficking. In Autophagy, 20, 809-829. doi:10.1080/15548627.2023.2274205. https://pubmed.ncbi.nlm.nih.gov/37876250/
2. Bi, Paul Y, Killackey, Samuel A, Schweizer, Linus, Girardin, Stephen E. 2023. NLRX1: Versatile functions of a mitochondrial NLR protein that controls mitophagy. In Biomedical journal, 47, 100635. doi:10.1016/j.bj.2023.100635. https://pubmed.ncbi.nlm.nih.gov/37574163/
3. Nagai-Singer, Margaret A, Morrison, Holly A, Allen, Irving C. 2019. NLRX1 Is a Multifaceted and Enigmatic Regulator of Immune System Function. In Frontiers in immunology, 10, 2419. doi:10.3389/fimmu.2019.02419. https://pubmed.ncbi.nlm.nih.gov/31681307/
4. Liu, Ye, Kors, Lotte, Butter, Loes M, Florquin, Sandrine, Tammaro, Alessandra. 2023. NLRX1 Prevents M2 Macrophage Polarization and Excessive Renal Fibrosis in Chronic Obstructive Nephropathy. In Cells, 13, . doi:10.3390/cells13010023. https://pubmed.ncbi.nlm.nih.gov/38201227/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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