C57BL/6NCya-Cep295em1/Cya
Common Name:
Cep295-KO
Product ID:
S-KO-09180
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Contact for Pricing
Basic Information
Strain Name
Cep295-KO
Strain ID
KOCMP-319675-Cep295-B6N-VA
Gene Name
Product ID
S-KO-09180
Gene Alias
5830418K08Rik; 5832426L23Rik; Gm1131; Kiaa1731
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Cep295em1/Cya mice (Catalog S-KO-09180) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000098979
NCBI RefSeq
NM_176976
Target Region
Exon 2~6
Size of Effective Region
~7.8 kb
Detailed Document
Overview of Gene Research
CEP295, also known as Ana1 in Drosophila, is an essential protein for centrosome and centriole maintenance. Centrosomes and centrioles are microtubule-organizing centers (MTOC) with diverse functions. CEP295 is involved in pathways related to centriole elongation, centriole-to-centrosome conversion, and maintaining centrosome structural integrity [1,2,3,6]. Its proper function is crucial for normal cell division, cilia formation, and overall cellular development, and genetic models are valuable for studying its functions.
Depletion of CEP295 in cell lines and patient-derived fibroblasts leads to a decrease in centriole and centrosome numbers, triggering p53-dependent G1 cell cycle arrest [4]. It is required for building the distal half centrioles and post-translational modification of centriolar microtubules; without it, the recruitments of POC5 and POC1B to the distal half centrioles are inhibited, resulting in shorter centrioles [2]. In the absence of PPP1R35, nascent centrioles cannot recruit CEP295, and they become disintegrated after mitosis [5]. Also, CEP295 is necessary for the conversion of daughter centrioles into functional mother centrioles, acting upstream of the conserved pathway for centriole formation [3].
In conclusion, CEP295 is vital for centriole and centrosome-related biological functions such as centriole elongation, conversion, and maintenance of structural integrity. Model-based research, especially loss-of-function experiments, has revealed its significance. Bi-allelic variants in CEP295 cause Seckel-like syndrome in humans, characterized by primary microcephaly, developmental delay, intellectual disability, short stature, and craniofacial and digital abnormalities [4].
References:
1. Pimenta-Marques, Ana, Perestrelo, Tania, Reis-Rodrigues, Patricia, Lince-Faria, Mariana, Bettencourt-Dias, Mónica. 2024. Ana1/CEP295 is an essential player in the centrosome maintenance program regulated by Polo kinase and the PCM. In EMBO reports, 25, 102-127. doi:10.1038/s44319-023-00020-6. https://pubmed.ncbi.nlm.nih.gov/38200359/
2. Chang, Ching-Wen, Hsu, Wen-Bin, Tsai, Jhih-Jie, Tang, Chieh-Ju C, Tang, Tang K. 2016. CEP295 interacts with microtubules and is required for centriole elongation. In Journal of cell science, 129, 2501-13. doi:10.1242/jcs.186338. https://pubmed.ncbi.nlm.nih.gov/27185865/
3. Tsuchiya, Yuki, Yoshiba, Satoko, Gupta, Akshari, Watanabe, Koki, Kitagawa, Daiju. 2016. Cep295 is a conserved scaffold protein required for generation of a bona fide mother centriole. In Nature communications, 7, 12567. doi:10.1038/ncomms12567. https://pubmed.ncbi.nlm.nih.gov/27562453/
4. Li, Niu, Xu, Yufei, Chen, Hongzhu, Machol, Keren, Wang, Jian. 2023. Bi-allelic variants in CEP295 cause Seckel-like syndrome presenting with primary microcephaly, developmental delay, intellectual disability, short stature, craniofacial and digital abnormalities. In EBioMedicine, 99, 104940. doi:10.1016/j.ebiom.2023.104940. https://pubmed.ncbi.nlm.nih.gov/38154379/
5. Fong, Chii Shyang, Ozaki, Kanako, Tsou, Meng-Fu Bryan. 2018. PPP1R35 ensures centriole homeostasis by promoting centriole-to-centrosome conversion. In Molecular biology of the cell, 29, 2801-2808. doi:10.1091/mbc.E18-08-0525. https://pubmed.ncbi.nlm.nih.gov/30230954/
6. Izquierdo, Denisse, Wang, Won-Jing, Uryu, Kunihiro, Tsou, Meng-Fu Bryan. 2014. Stabilization of cartwheel-less centrioles for duplication requires CEP295-mediated centriole-to-centrosome conversion. In Cell reports, 8, 957-65. doi:10.1016/j.celrep.2014.07.022. https://pubmed.ncbi.nlm.nih.gov/25131205/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen