C57BL/6JCya-Rinlem1/Cya
Common Name
Rinl-KO
Product ID
S-KO-09287
Backgroud
C57BL/6JCya
Strain ID
KOCMP-320435-Rinl-B6J-VA
When using this mouse strain in a publication, please cite “Rinl-KO Mouse (Catalog S-KO-09287) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Rinl-KO
Strain ID
KOCMP-320435-Rinl-B6J-VA
Gene Name
Product ID
S-KO-09287
Gene Alias
5830482F20Rik, 9930116N10Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 7
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000059857
NCBI RefSeq
NM_177158
Target Region
Exon 4
Size of Effective Region
~1.1 kb
Overview of Gene Research
Rinl, also known as Ras and Rab interactor (or Ras interaction/interference)-like, is a guanine nucleotide-exchange factor (GEF) for the Rab5 subfamily. The Rab family of small GTPases is crucial in membrane trafficking, and Rinl, with its conserved VPS9 domain, drives the active GTP-bound state of Rab5 subfamily proteins. It is involved in multiple biological processes such as endocytosis and is associated with pathways like the EphA8-degradation pathway and CD28-related signaling pathways [1,2,3].
Loss-of-function studies have provided insights into Rinl's role. In mice, loss of Rinl leads to an increase of T follicular helper (Tfh) cells during aging, upon in vivo immunization, and acute LCMV Armstrong infection, highlighting its role as a negative regulator of Tfh generation. Mechanistically, Rinl regulates CD28 internalization and signaling, which shapes CD4+ T cell activation and differentiation [2].
In conclusion, Rinl is an important GEF that plays a role in membrane-associated processes and cell-signaling pathways. The gene knockout mouse models have revealed its significance in Tfh cell differentiation, emphasizing its potential importance in immunological-related disease areas.
References:
1. Kajiho, Hiroaki, Fukushima, Shinichi, Kontani, Kenji, Katada, Toshiaki. 2012. RINL, guanine nucleotide exchange factor Rab5-subfamily, is involved in the EphA8-degradation pathway with odin. In PloS one, 7, e30575. doi:10.1371/journal.pone.0030575. https://pubmed.ncbi.nlm.nih.gov/22291991/
2. Sandner, Lisa, Alteneder, Marlis, Rica, Ramona, Herbst, Ruth, Boucheron, Nicole. 2023. The guanine nucleotide exchange factor Rin-like controls Tfh cell differentiation via CD28 signaling. In The Journal of experimental medicine, 220, . doi:10.1084/jem.20221466. https://pubmed.ncbi.nlm.nih.gov/37703004/
3. Woller, Barbara, Luiskandl, Susan, Popovic, Milica, Rehmann, Holger, Herbst, Ruth. 2011. Rin-like, a novel regulator of endocytosis, acts as guanine nucleotide exchange factor for Rab5a and Rab22. In Biochimica et biophysica acta, 1813, 1198-210. doi:10.1016/j.bbamcr.2011.03.005. https://pubmed.ncbi.nlm.nih.gov/21419809/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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