C57BL/6JCya-Dctdem1/Cya
Common Name
Dctd-KO
Product ID
S-KO-09330
Backgroud
C57BL/6JCya
Strain ID
KOCMP-320685-Dctd-B6J-VA
Status
When using this mouse strain in a publication, please cite “Dctd-KO Mouse (Catalog S-KO-09330) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Dctd-KO
Strain ID
KOCMP-320685-Dctd-B6J-VA
Gene Name
Product ID
S-KO-09330
Gene Alias
6030466N05Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 8
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000170263
NCBI RefSeq
NM_178788
Target Region
Exon 2~3
Size of Effective Region
~0.4 kb
Overview of Gene Research
Dctd, short for dCMP deaminase, is an enzyme with significant functions in nucleotide metabolism. It is involved in the conversion of dCMP to dUMP, a crucial step in nucleotide biosynthesis pathways [1].
In the context of cancer treatment, genome-scale CRISPR/Cas9 screens have shown that loss of DctD causes resistance to the nucleoside analogue decitabine (5-aza-dC) used in treating several haematological cancers. This indicates that DctD-mediated generation of 5-aza-dUMP is cytotoxic, suggesting that DctD plays a role in the efficacy of decitabine-based cancer therapies [1].
In conclusion, DctD is essential for the cytotoxic effects of decitabine in cancer treatment. Gene-knockout models, like those used in the CRISPR/Cas9 screens, have revealed its role in determining drug sensitivity, which may help in identifying predictive biomarkers for decitabine treatment and improving the treatment of haematological cancers.
References:
1. Carnie, Christopher J, Götz, Maximilian J, Palma-Chaundler, Chloe S, Stingele, Julian, Jackson, Stephen P. 2024. Decitabine cytotoxicity is promoted by dCMP deaminase DCTD and mitigated by SUMO-dependent E3 ligase TOPORS. In The EMBO journal, 43, 2397-2423. doi:10.1038/s44318-024-00108-2. https://pubmed.ncbi.nlm.nih.gov/38760575/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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