C57BL/6JCya-Thsd7aem1/Cya
Common Name
Thsd7a-KO
Product ID
S-KO-09508
Backgroud
C57BL/6JCya
Strain ID
KOCMP-330267-Thsd7a-B6J-VA
When using this mouse strain in a publication, please cite “Thsd7a-KO Mouse (Catalog S-KO-09508) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Thsd7a-KO
Strain ID
KOCMP-330267-Thsd7a-B6J-VA
Gene Name
Product ID
S-KO-09508
Gene Alias
Gm837
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 6
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000119581
NCBI RefSeq
NM_001164805
Target Region
Exon 2
Size of Effective Region
~2.1 kb
Overview of Gene Research
Thrombospondin domain-containing 7A (Thsd7a) is a large transmembrane glycoprotein expressed by podocytes. Its exact function remains speculative, but some features suggest a role in adhesion. In the context of membranous nephropathy (MN), an autoimmune kidney disease, Thsd7a has emerged as a significant autoantigen [2].
In MN, about 3-5% of primary cases are associated with anti-Thsd7a antibodies [1]. The presence of these autoantibodies is used for MN diagnosis, and their levels correlate with disease severity, serving as biomarker for monitoring disease progression and treatment response [1]. A recent study developed a mouse model of Thsd7a-associated MN using a commercially available antibody. This model showed that Thsd7a-associated MN involves both complement-mediated and autonomous podocyte injury. Complement depletion only partially attenuated proteinuria and glomerular injury, indicating that there are also complement-independent pathomechanisms. In vitro, anti-Thsd7a antibody exposure caused podocytopathic changes in primary podocytes [3].
In conclusion, Thsd7a, as an autoantigen expressed by podocytes, plays a crucial role in the pathogenesis of membranous nephropathy. The Thsd7a-associated MN mouse model has provided valuable insights into the complex mechanisms of podocyte injury in this disease, which may contribute to the development of novel treatments [3].
References:
1. Gu, Yan, Xu, Hui, Tang, Damu. 2021. Mechanisms of Primary Membranous Nephropathy. In Biomolecules, 11, . doi:10.3390/biom11040513. https://pubmed.ncbi.nlm.nih.gov/33808418/
2. Beck, Laurence H. 2017. PLA2R and THSD7A: Disparate Paths to the Same Disease? In Journal of the American Society of Nephrology : JASN, 28, 2579-2589. doi:10.1681/ASN.2017020178. https://pubmed.ncbi.nlm.nih.gov/28674044/
3. Liu, Jing, Malhotra, Deepak, Ge, Yan, Dworkin, Lance, Gong, Rujun. 2024. THSD7A-associated membranous nephropathy involves both complement-mediated and autonomous podocyte injury. In Frontiers in pharmacology, 15, 1430451. doi:10.3389/fphar.2024.1430451. https://pubmed.ncbi.nlm.nih.gov/39086386/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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