C57BL/6JCya-Fan1em1/Cya
Common Name:
Fan1-KO
Product ID:
S-KO-09528
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Fan1-KO
Strain ID
KOCMP-330554-Fan1-B6J-VA
Gene Name
Product ID
S-KO-09528
Gene Alias
6030441H18Rik; Mtmr15; mFAN1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fan1em1/Cya mice (Catalog S-KO-09528) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000163289
NCBI RefSeq
NM_177893
Target Region
Exon 1~5
Size of Effective Region
~7.5 kb
Detailed Document
Overview of Gene Research
FAN1, short for FANCD2/FANCI-associated nuclease, encodes a DNA repair nuclease. It is involved in DNA interstrand cross-link repair and is associated with pathways related to maintaining chromosomal stability. Genetic variations in FAN1 have been linked to various diseases, such as karyomegalic interstitial nephritis, 15q13.3 microdeletion/microduplication syndrome (associated with autism, schizophrenia, and epilepsy), cancer, and repeat expansion diseases [1].
In HD and FMR1 transgenic mice, Fan1 is required to suppress high levels of ongoing somatic CAG and CGG repeat expansions respectively [1]. In human cell extracts, FAN1 can remove triplet repeat extrusions via a PCNA-and RFC-dependent mechanism, preventing repeat expansion [2]. FAN1 also controls mismatch repair complex assembly by binding to MLH1, thus stabilizing CAG repeat expansion in HD [3]. Disruption of the FAN1-MLH1 interaction leads to cellular hypersensitivity to ICL damage and defective repair of CAG/CTG slip-outs [4].
In conclusion, FAN1 plays a crucial role in DNA repair, especially in preventing repeat expansions. Studies using transgenic mouse models have revealed its significance in diseases like Huntington's disease. Understanding FAN1's functions may offer potential therapeutic targets for treating related diseases.
References:
1. Deshmukh, Amit L, Porro, Antonio, Mohiuddin, Mohiuddin, Sartori, Alessandro A, Pearson, Christopher E. . FAN1, a DNA Repair Nuclease, as a Modifier of Repeat Expansion Disorders. In Journal of Huntington's disease, 10, 95-122. doi:10.3233/JHD-200448. https://pubmed.ncbi.nlm.nih.gov/33579867/
2. Phadte, Ashutosh S, Bhatia, Mayuri, Ebert, Hope, Komolov, Konstantin E, Pluciennik, Anna. 2023. FAN1 removes triplet repeat extrusions via a PCNA- and RFC-dependent mechanism. In Proceedings of the National Academy of Sciences of the United States of America, 120, e2302103120. doi:10.1073/pnas.2302103120. https://pubmed.ncbi.nlm.nih.gov/37549289/
3. Goold, Robert, Hamilton, Joseph, Menneteau, Thomas, Balmus, Gabriel, Tabrizi, Sarah J. . FAN1 controls mismatch repair complex assembly via MLH1 retention to stabilize CAG repeat expansion in Huntington's disease. In Cell reports, 36, 109649. doi:10.1016/j.celrep.2021.109649. https://pubmed.ncbi.nlm.nih.gov/34469738/
4. Porro, Antonio, Mohiuddin, Mohiuddin, Zurfluh, Christina, Pearson, Christopher E, Sartori, Alessandro A. 2021. FAN1-MLH1 interaction affects repair of DNA interstrand cross-links and slipped-CAG/CTG repeats. In Science advances, 7, . doi:10.1126/sciadv.abf7906. https://pubmed.ncbi.nlm.nih.gov/34330701/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen