C57BL/6JCya-Zfp36l3em1/Cya
Common Name:
Zfp36l3-KO
Product ID:
S-KO-09587
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Zfp36l3-KO
Strain ID
KOCMP-333473-Zfp36l3-B6J-VA
Gene Name
Product ID
S-KO-09587
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
X
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zfp36l3em1/Cya mice (Catalog S-KO-09587) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000071711
NCBI RefSeq
NM_001009549
Target Region
Exon 1
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Zfp36l3 is a member of the zinc finger protein 36 (ZFP36) family, which is involved in post-transcriptional regulation of messenger RNA (mRNA) metabolism [1]. It contains two tandemly repeated CCCH-type zinc-finger motifs and binds to adenine uridine-rich elements in the 3'-untranslated regions of specific mRNA, leading to target mRNA decay [1]. This gene is unique to rodents and is located on the X chromosome [2,3]. Zfp36l3 is particularly important for placental and extra-embryonic tissue function, suggesting its significance in female reproductive physiology and overall fecundity [2-4]. Genetic models, especially mouse models, are valuable for studying Zfp36l3's functions.
In Zfp36l3 knockout (KO) mice, neonatal survival rates decreased, indicating its importance for offspring survival [4]. RNA-Seq analysis of placental mRNA from KO mice showed many up-regulated transcripts, suggesting these are potential ZFP36L3 target mRNAs [4]. Among them, the type 1 transferrin receptor mRNA was unexpectedly decreased in KO placentas, despite increased stability in KO stem cells [4]. Since this receptor is crucial for placental iron uptake, and its decrease was accompanied by decreased iron stores in the KO fetus, Zfp36l3 may be linked to placental iron metabolism [4].
In conclusion, Zfp36l3 is a rodent-specific gene playing essential roles in placental function, female fecundity, and potentially placental iron metabolism as revealed by KO mouse models. Understanding Zfp36l3 contributes to insights into rodent reproductive biology and potential implications for intrauterine deficiencies that may have long-term consequences.
References:
1. Makita, Sohei, Takatori, Hiroaki, Nakajima, Hiroshi. 2021. Post-Transcriptional Regulation of Immune Responses and Inflammatory Diseases by RNA-Binding ZFP36 Family Proteins. In Frontiers in immunology, 12, 711633. doi:10.3389/fimmu.2021.711633. https://pubmed.ncbi.nlm.nih.gov/34276705/
2. Gingerich, Timothy J, Stumpo, Deborah J, Lai, Wi S, Steppan, Scott J, Blackshear, Perry J. 2015. Emergence and evolution of Zfp36l3. In Molecular phylogenetics and evolution, 94, 518-530. doi:10.1016/j.ympev.2015.10.016. https://pubmed.ncbi.nlm.nih.gov/26493225/
3. Blackshear, Perry J, Phillips, Ruth S, Ghosh, Sanjukta, Richfield, Eric K, Lai, Wi S. 2005. Zfp36l3, a rodent X chromosome gene encoding a placenta-specific member of the Tristetraprolin family of CCCH tandem zinc finger proteins. In Biology of reproduction, 73, 297-307. doi:. https://pubmed.ncbi.nlm.nih.gov/15814898/
4. Stumpo, Deborah J, Trempus, Carol S, Tucker, Charles J, Bortner, Donna M, Blackshear, Perry J. 2016. Deficiency of the placenta- and yolk sac-specific tristetraprolin family member ZFP36L3 identifies likely mRNA targets and an unexpected link to placental iron metabolism. In Development (Cambridge, England), 143, 1424-33. doi:10.1242/dev.130369. https://pubmed.ncbi.nlm.nih.gov/26952984/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen