C57BL/6JCya-Cfap58em1/Cya
Common Name:
Cfap58-KO
Product ID:
S-KO-09727
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Cfap58-KO
Strain ID
KOCMP-381229-Cfap58-B6J-VA
Gene Name
Product ID
S-KO-09727
Gene Alias
Ccdc147; Gm969
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
19
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cfap58em1/Cya mice (Catalog S-KO-09727) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000066308
NCBI RefSeq
NM_001163267
Target Region
Exon 2~3
Size of Effective Region
~3.1 kb
Detailed Document
Overview of Gene Research
Cfap58, or cilia and flagella associated protein 58, is a testis-enriched gene. It is crucial for sperm flagellogenesis and may be involved in the intra-manchette transport/intra-flagellar transport pathway. It also has potential connections with the Notch signaling pathway in ciliogenesis [1,3].
Gene knockout mouse models have shown that loss of Cfap58 leads to severe sperm tail defects, abnormal sperm head shaping due to manchette structure impairment, and increased spermatozoa apoptosis. Biochemically, it interacts with IFT88 and CCDC42 and may stabilize other cargo proteins like CCDC42 in relevant transport pathways [1]. In humans, biallelic mutations or loss-of-function variants in CFAP58 are associated with multiple morphological abnormalities of the sperm flagella (MMAF), a severe form of asthenoteratozoospermia, presenting with flagellar axoneme and mitochondrial sheath defects [2,4]. Exome sequencing in patients with severe sperm motility disorders also identified a patient with a pathogenic variant in CFAP58 [5].
In summary, Cfap58 is essential for spermatogenesis. The study of Cfap58 knockout mouse models has provided crucial insights into male infertility caused by MMAF-associated asthenoteratozoospermia, highlighting the gene's significance in sperm development and male reproductive health [1,2,4,5].
References:
1. Wei, Xiaochao, Wang, Xiuge, Yang, Chunhong, Gao, Yundong, Huang, Jinming. 2024. CFAP58 is involved in the sperm head shaping and flagellogenesis of cattle and mice. In Development (Cambridge, England), 151, . doi:10.1242/dev.202608. https://pubmed.ncbi.nlm.nih.gov/38602507/
2. Sha, Yankun, Sha, Yanwei, Liu, Wensheng, Wang, Yifeng, Zhou, Huiliang. 2020. Biallelic mutations of CFAP58 are associated with multiple morphological abnormalities of the sperm flagella. In Clinical genetics, 99, 443-448. doi:10.1111/cge.13898. https://pubmed.ncbi.nlm.nih.gov/33314088/
3. Li, Zheng-Zheng, Zhao, Wen-Long, Wang, Gui-Shuan, Gu, Ni-Hao, Sun, Fei. . The novel testicular enrichment protein Cfap58 is required for Notch-associated ciliogenesis. In Bioscience reports, 40, . doi:10.1042/BSR20192666. https://pubmed.ncbi.nlm.nih.gov/31904090/
4. He, Xiaojin, Liu, Chunyu, Yang, Xiaoyu, Zhang, Feng, Cao, Yunxia. 2020. Bi-allelic Loss-of-function Variants in CFAP58 Cause Flagellar Axoneme and Mitochondrial Sheath Defects and Asthenoteratozoospermia in Humans and Mice. In American journal of human genetics, 107, 514-526. doi:10.1016/j.ajhg.2020.07.010. https://pubmed.ncbi.nlm.nih.gov/32791035/
5. Oud, M S, Houston, B J, Volozonoka, L, Chemes, H E, Sheth, H. . Exome sequencing reveals variants in known and novel candidate genes for severe sperm motility disorders. In Human reproduction (Oxford, England), 36, 2597-2611. doi:10.1093/humrep/deab099. https://pubmed.ncbi.nlm.nih.gov/34089056/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen