C57BL/6NCya-Hjurpem1/Cya
Common Name:
Hjurp-KO
Product ID:
S-KO-09734
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Hjurp-KO
Strain ID
KOCMP-381280-Hjurp-B6N-VA
Gene Name
Product ID
S-KO-09734
Gene Alias
6430706D22Rik; A730008H23Rik; C330011F01Rik; Gm975; Mfleg1
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Hjurpem1/Cya mice (Catalog S-KO-09734) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000054674
NCBI RefSeq
NM_198652
Target Region
Exon 3~6
Size of Effective Region
~7.3 kb
Detailed Document
Overview of Gene Research
HJURP, short for Holliday Junction Recognition Protein, is a highly specialized mitogenic protein and a chaperone protein of histone H3. The HJURP gene is located on chromosome 2q37.1 and is involved in nucleosome composition in the mitotic region, forming a three-dimensional crystal structure with Centromere Protein A (CENP-A) and the histone 4 complex. It is associated with pathways like PRDX1/reactive oxygen species (ROS) pathway and is important in processes such as cell proliferation, DNA repair, and centromere-kinetochore recruitment [2].
In prostate cancer cells, HJURP inhibits sensitivity to ferroptosis inducers by enhancing the peroxidase activity of PRDX1 through forming disulfide-linked intermediates with PRDX1 [1]. In hepatocellular carcinoma, HJURP promotes Epithelial-to-Mesenchymal Transition via upregulating SPHK1, affecting cell migration and invasion [3]. In ovarian cancer, HJURP promotes tumor progression and chemoresistance, and its knockdown inhibits proliferation and metastasis [4]. In triple-negative breast cancer, HJURP regulates cell proliferation and chemo-resistance via the YAP1/NDRG1 transcriptional axis [5]. In glioma cells, HJURP is recruited to double-strand break (DSB) sites, facilitating DNA repair by promoting chromatin reorganization [6].
In conclusion, HJURP plays crucial roles in multiple biological processes and diseases, especially in cancer. Studies, although not specifically from KO/CKO mouse models in the provided references, have shown its involvement in cancer cell proliferation, metastasis, chemoresistance, and DNA repair. Understanding HJURP may provide new therapeutic targets for various cancers.
References:
1. Lai, Wenjie, Zhu, Weian, Wu, Jianjie, Qiu, Xiaofu, Wen, Xingqiao. 2024. HJURP inhibits sensitivity to ferroptosis inducers in prostate cancer cells by enhancing the peroxidase activity of PRDX1. In Redox biology, 77, 103392. doi:10.1016/j.redox.2024.103392. https://pubmed.ncbi.nlm.nih.gov/39405980/
2. Li, Lin, Yuan, Qiang, Chu, Yue-Ming, Huo, Dan-Qun, Zhang, Xiao-Fen. 2023. Advances in holliday junction recognition protein (HJURP): Structure, molecular functions, and roles in cancer. In Frontiers in cell and developmental biology, 11, 1106638. doi:10.3389/fcell.2023.1106638. https://pubmed.ncbi.nlm.nih.gov/37025176/
3. Chen, Tianchi, Zhou, Lingfeng, Zhou, Yuan, Zhou, Lin, Zheng, Shusen. 2019. HJURP Promotes Epithelial-to-Mesenchymal Transition via Upregulating SPHK1 in Hepatocellular Carcinoma. In International journal of biological sciences, 15, 1139-1147. doi:10.7150/ijbs.30904. https://pubmed.ncbi.nlm.nih.gov/31223275/
4. Dou, Zhiyuan, Qiu, Chunping, Zhang, Xun, Song, Kun, Kong, Beihua. 2022. HJURP Promotes Malignant Progression and Mediates Sensitivity to Cisplatin and WEE1-inhibitor in Serous Ovarian Cancer. In International journal of biological sciences, 18, 1188-1210. doi:10.7150/ijbs.65589. https://pubmed.ncbi.nlm.nih.gov/35173547/
5. Mao, Misha, Jia, Yunlu, Chen, Yongxia, Ju, Siwei, Wang, Linbo. 2022. HJURP regulates cell proliferation and chemo-resistance via YAP1/NDRG1 transcriptional axis in triple-negative breast cancer. In Cell death & disease, 13, 396. doi:10.1038/s41419-022-04833-6. https://pubmed.ncbi.nlm.nih.gov/35459269/
6. Serafim, Rodolfo B, Cardoso, Cibele, Storti, Camila B, Price, Brendan D, Valente, Valeria. 2024. HJURP is recruited to double-strand break sites and facilitates DNA repair by promoting chromatin reorganization. In Oncogene, 43, 804-820. doi:10.1038/s41388-024-02937-1. https://pubmed.ncbi.nlm.nih.gov/38279062/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen